Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0000922
UniProt IDP60228
Primary gene name(s)EIF3E
Synonym gene name(s)EIF3S6, INT6
Protein nameEukaryotic translation initiation factor 3 subunit E
Protein functionComponent of the eukaryotic translation initiation factor 3, eIF-3 complex, which is required for several steps in the initiation of protein synthesis, PubMed:17581632, PubMed:25849773, PubMed:27462815. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex, 43S PIC. The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation, PubMed:17581632. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression, PubMed:25849773. Required for nonsense-mediated mRNA decay, NMD; may act in conjunction with UPF2 to divert mRNAs from translation to the NMD pathway, PubMed:17468741. May interact with MCM7 and EPAS1 and regulate the proteasome-mediated degradation of these proteins, PubMed:17310990, PubMed:17324924. {ECO:0000255|HAMAP-Rule:MF_03004, ECO:0000269|PubMed:17310990, ECO:0000269|PubMed:17324924, ECO:0000269|PubMed:17468741, ECO:0000269|PubMed:17581632, ECO:0000269|PubMed:25849773, ECO:0000269|PubMed:27462815}.
Subcellular locationCytoplasm. Nucleus, PML body.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P60228
Gene Ontology
(Biological Process)
Complete annatation
formation of translation preinitiation complex [GO:0001731];
negative regulation of translational initiation [GO:0045947];
nuclear-transcribed mRNA catabolic process, nonsense-mediated decay [GO:0000184];
positive regulation of mRNA binding [GO:1902416];
positive regulation of translation [GO:0045727];
regulation of translational initiation [GO:0006446];
translational initiation [GO:0006413]
Gene Ontology
(Molecular Function)
Complete annatation
poly(A RNA binding [GO:0044822];
protein N-terminus binding [GO:0047485];
translation initiation factor activity [GO:0003743]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasm [GO:0005737];
cytosol [GO:0005829];
eukaryotic 43S preinitiation complex [GO:0016282];
eukaryotic 48S preinitiation complex [GO:0033290];
eukaryotic translation initiation factor 3 complex [GO:0005852];
extracellular exosome [GO:0070062];
membrane [GO:0016020];
nucleoplasm [GO:0005654];
nucleus [GO:0005634];
PML body [GO:0016605]
Protein-protein interaction109857
Phylogenetic treeP60228
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.02997225825682990.9270044753927570.95266681120412
AZA vs. DISU-0.1556626932041690.537296400043390.941913020997389
AZA vs. IL7-0.03377438788861640.8890521958379220.999311006273513
AZA vs. SAHA-0.01142548643440850.9625290693569770.990779256780515
DISU vs. CD3-0.1385299012822770.7018642082410780.791285793855811
DISU vs. IL70.1133944739921790.6517379010104090.898979674053611
DISU vs. SAHA0.1440424054963460.6204390364163220.880275465058394
DMSO vs. AZA-0.1985559278361160.3086356425867111
DMSO vs. CD3-0.1840868483579180.565196968780060.666877532477413
DMSO vs. DISU-0.04598232878969470.8502379935798380.982583769983649
DMSO vs. IL70.1725494143075260.4086152676153130.847494592253841
DMSO vs. SAHA0.180257052554710.4432630838971170.77580048048002
HIV vs. Mock in Activation-0.3689605604643120.5530893286936940.999983755607037
HIV vs. Mock in Latency-0.1991131071143550.2253116744183990.999834320637052
IL7 vs. CD30.006373381547967620.9841377438303230.990181444012714
SAHA vs. CD3-0.007890885460431450.9821807234851830.987883835208017
SAHA vs. IL70.01731937611760690.9431721723707130.978024358224409
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.857779 0.000305467
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.985 0.918 0.816 0.701 0.861
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
3J8B EM - E=1-395.
3J8C EM - E=1-395.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Envelope surface glycoprotein gp120 complexes with 23125841
Gag-Pol complexes with 23125841
Pol interacts with 22190034
Pr55(Gag) complexes with 23125841
retropepsin interacts with 22190034
Nef complexes with 23125841

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa03013 RNA transport - Homo sapiens (human)
hsa05160 Hepatitis C - Homo sapiens (human)