Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0000920
UniProt IDQ14152
Primary gene name(s)EIF3A
Synonym gene name(s)EIF3S10, KIAA0139
Protein nameEukaryotic translation initiation factor 3 subunit A
Protein functionRNA-binding component of the eukaryotic translation initiation factor 3, eIF-3 complex, which is required for several steps in the initiation of protein synthesis, PubMed:17581632, PubMed:25849773. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex, 43S PIC. The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation, PubMed:17581632, PubMed:11169732. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression, PubMed:25849773, PubMed:27462815. {ECO:0000255|HAMAP-Rule:MF_03000, ECO:0000269|PubMed:11169732, ECO:0000269|PubMed:17581632, ECO:0000269|PubMed:25849773, ECO:0000269|PubMed:27462815}.; FUNCTION:, Microbial infection Essential for the initiation of translation on type-1 viral ribosomal entry sites, IRESs, like for HCV, PV, EV71 or BEV translation, PubMed:23766293, PubMed:24357634. {ECO:0000269|PubMed:23766293, ECO:0000269|PubMed:24357634}.; FUNCTION:, Microbial infection In case of FCV infection, plays a role in the ribosomal termination-reinitiation event leading to the translation of VP2, PubMed:18056426. {ECO:0000269|PubMed:18056426}.
Subcellular locationCytoplasm {ECO:0000255|HAMAP-Rule:MF_03000, ECO:0000269|PubMed:9150439}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q14152
Gene Ontology
(Biological Process)
Complete annatation
formation of cytoplasmic translation initiation complex [GO:0001732];
formation of translation preinitiation complex [GO:0001731];
IRES-dependent viral translational initiation [GO:0075522];
negative regulation of ERK1 and ERK2 cascade [GO:0070373];
regulation of translational initiation [GO:0006446];
translational initiation [GO:0006413];
translation reinitiation [GO:0002188];
viral translational termination-reinitiation [GO:0075525]
Gene Ontology
(Molecular Function)
Complete annatation
poly(A RNA binding [GO:0044822];
structural molecule activity [GO:0005198];
translation initiation factor activity [GO:0003743]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasm [GO:0005737];
cytosol [GO:0005829];
eukaryotic 43S preinitiation complex [GO:0016282];
eukaryotic 48S preinitiation complex [GO:0033290];
eukaryotic translation initiation factor 3 complex [GO:0005852];
eukaryotic translation initiation factor 3 complex, eIF3m [GO:0071541];
membrane [GO:0016020];
nucleolus [GO:0005730];
nucleus [GO:0005634]
Protein-protein interaction114210
Phylogenetic treeQ14152
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.7077979300909540.03108103840109090.0670080690575371
AZA vs. DISU-0.07759898840945610.7584325168426670.976021571236163
AZA vs. IL70.2762306758685020.1496462932101580.917267178119725
AZA vs. SAHA-0.1822358383308490.4538635923527840.795502627965502
DISU vs. CD3-0.7978804636776170.02839314700978490.0684249876939245
DISU vs. IL70.345016690930730.1701547512502480.534395228587608
DISU vs. SAHA-0.104041001028530.7204660861755690.917103213595783
DMSO vs. AZA-0.01533436566405610.9267220762292381
DMSO vs. CD3-0.7338875257955920.02215770907416050.0487806352772509
DMSO vs. DISU0.06051727282803770.8035797366595170.977285200264502
DMSO vs. IL70.2987782467686090.09551037994529310.566734231787423
DMSO vs. SAHA-0.1747503405479690.4574086239947910.786851429314632
HIV vs. Mock in Activation0.1709522949182070.7832978894357680.999983755607037
HIV vs. Mock in Latency-0.01338282763731430.9350649404499660.999834320637052
IL7 vs. CD3-0.4230445694311040.1879324004832940.30766862957366
SAHA vs. CD3-0.9156505133093970.009982339411013340.0256137687526655
SAHA vs. IL7-0.4625927483783650.05739074425404130.20118792532219
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.0455855 0.852954
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.998 0.941 0.818 0.72 0.932
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
3J8B EM - A=1-494.
3J8C EM - A=1-494.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Envelope surface glycoprotein gp120 complexes with 23125841
Gag-Pol complexes with 23125841
Pol interacts with 22190034
Pr55(Gag) complexes with 23125841
retropepsin interacts with 22190034
Nef complexes with 23125841

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa03013 RNA transport - Homo sapiens (human)