Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0000919
UniProt IDP41091
Primary gene name(s)EIF2S3
Synonym gene name(s)EIF2G
Protein nameEukaryotic translation initiation factor 2 subunit 3
Protein functionAs a subunit of eukaryotic initiation factor 2, eIF2, involved in the early steps of protein synthesis. In the presence of GTP, eIF2 forms a ternary complex with initiator tRNA Met-tRNAi and then recruits the 40S ribosomal complex, a step that determines the rate of protein translation. This step is followed by mRNA binding to form the 43S pre-initiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF2 and release of an eIF2-GDP binary complex. In order for eIF2 to recycle and catalyze another round of initiation, the GDP bound to eIF2 must exchange with GTP by way of a reaction catalyzed by eIF2B, By similarity. Along with its paralog on chromosome Y, may contribute to spermatogenesis up to the round spermatid stage, By similarity. {ECO:0000250, ECO:0000250|UniProtKB:Q9Z0N1}.
Subcellular locationunknown
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P41091
Gene Ontology
(Biological Process)
Complete annatation
formation of translation preinitiation complex [GO:0001731];
translational initiation [GO:0006413];
transmembrane transport [GO:0055085]
Gene Ontology
(Molecular Function)
Complete annatation
GTPase activity [GO:0003924];
GTP binding [GO:0005525];
translation factor activity, RNA binding [GO:0008135];
translation initiation factor activity [GO:0003743]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasm [GO:0005737];
cytosol [GO:0005829];
extracellular exosome [GO:0070062]
Protein-protein interaction108287
Phylogenetic treeP41091
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.4108089314792690.2094814227857070.316046446869487
AZA vs. DISU-0.004315068107396890.9863642940795840.998932793566471
AZA vs. IL70.1601393127500280.4038383231057260.999311006273513
AZA vs. SAHA0.1223670152093970.6160423351870.875360859609696
DISU vs. CD3-0.4274906042388430.2383726154533550.363356133617543
DISU vs. IL70.1555137315838150.5362332834064990.847294141111372
DISU vs. SAHA0.127563931180190.6622889093034470.897064653539346
DMSO vs. AZA-0.07652697762199620.6467976763664471
DMSO vs. CD3-0.4975870380419880.1199137142082240.197755895407432
DMSO vs. DISU-0.07376685594002740.7619455397040980.969857060964769
DMSO vs. IL70.2437400607197020.1743329704789150.684571380559948
DMSO vs. SAHA0.1913105296767020.4176701462680830.758033697121451
HIV vs. Mock in Activation-0.02129097108940150.9726879244986230.999983755607037
HIV vs. Mock in Latency-0.04103251286799690.8029953160197690.999834320637052
IL7 vs. CD3-0.2423490185719920.4502736325023370.584328408326106
SAHA vs. CD3-0.3135912699740520.3751765514240040.491815468855231
SAHA vs. IL7-0.0417535373834570.8642232045080180.943820462042602
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
-1.5 0.151854965 -2.1 0.016121962 -2.4 0.024748531
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.289301 0.029289
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.045 0.967 0.945 0.909 0.895
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

Drugbank ID Drug Name Drug Status Pharmacological Action Drug Action
DB04315 Guanosine-5&,39;-Diphosphate experimental unknown unknown

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Rev interacts with 22174317
Tat competes with 9079663
Tat upregulates 23166591
Tat interacts with 10648795

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa03013 RNA transport - Homo sapiens (human)