Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0000917
UniProt IDP05198
Primary gene name(s)EIF2S1
Synonym gene name(s)EIF2A
Protein nameEukaryotic translation initiation factor 2 subunit 1
Protein functionFunctions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.
Subcellular locationCytoplasmic granule {ECO:0000250}. Note=The cytoplasmic granules are stress granules which are a dense aggregation in the cytosol composed of proteins and RNAs that appear when the cell is under stress. Colocalizes with NANOS3 in the stress granules, By similarity. {ECO:0000250}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P05198
Gene Ontology
(Biological Process)
Complete annatation
aging [GO:0007568];
cellular response to amino acid starvation [GO:0034198];
cellular response to heat [GO:0034605];
cellular response to UV [GO:0034644];
negative regulation of guanyl-nucleotide exchange factor activity [GO:1905098];
negative regulation of translational initiation in response to stress [GO:0032057];
PERK-mediated unfolded protein response [GO:0036499];
positive regulation of neuron death [GO:1901216];
positive regulation of type B pancreatic cell apoptotic process [GO:2000676];
protein autophosphorylation [GO:0046777];
response to endoplasmic reticulum stress [GO:0034976];
response to manganese-induced endoplasmic reticulum stress [GO:1990737];
translational initiation [GO:0006413];
transmembrane transport [GO:0055085]
Gene Ontology
(Molecular Function)
Complete annatation
poly(A RNA binding [GO:0044822];
ribosome binding [GO:0043022];
translation initiation factor activity [GO:0003743]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasmic stress granule [GO:0010494];
cytosol [GO:0005829];
eukaryotic 48S preinitiation complex [GO:0033290];
eukaryotic translation initiation factor 2 complex [GO:0005850];
extracellular exosome [GO:0070062];
glial limiting end-foot [GO:0097451];
membrane [GO:0016020];
multi-eIF complex [GO:0043614];
nucleus [GO:0005634];
polysome [GO:0005844];
ribosome [GO:0005840];
translation initiation ternary complex [GO:0044207]
Protein-protein interaction108285
Phylogenetic treeP05198
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD31.981423188250115.02067976082543e-096.28502370059476e-08
AZA vs. DISU0.06537375243047570.7960424158590370.981946742545343
AZA vs. IL70.4009699517439990.03727957879267670.593077611390911
AZA vs. SAHA-0.2350425248858760.3357615873847230.708266186431194
DISU vs. CD3-1.928477044662032.63924224142009e-072.91030998881979e-06
DISU vs. IL70.3267129533958010.1949023206516680.569012184339081
DISU vs. SAHA-0.2996222559168870.3045427506365170.688326903845673
DMSO vs. AZA-0.07132554256541060.6706839532919721
DMSO vs. CD3-2.063171049776835.08425412881763e-106.94417846333977e-09
DMSO vs. DISU-0.1382864537812360.5709403227488260.930025594527682
DMSO vs. IL70.4794722247010950.007796098601456030.184539419348666
DMSO vs. SAHA-0.1709461272235470.4686612815267020.794685008109737
HIV vs. Mock in Activation-0.1818371334699020.7698018203642130.999983755607037
HIV vs. Mock in Latency-0.01287660387332160.9379780702817360.999834320637052
IL7 vs. CD3-1.572067013483971.67398913142325e-061.55561271134048e-05
SAHA vs. CD3-2.241609579061871.2491585543728e-092.03291786332592e-08
SAHA vs. IL7-0.6394792328665410.008899240467385550.0575554498798923
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
-0.4153 0.02965

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.173229 0.223028
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.024 0.937 0.937 0.907 0.89
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
0.56 <0.0001 0.47 0.0013 0.07 0.0044 Protein Synthesis at 4; 8; and 20 hip
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
201144_s_at 1.46 No upregulated in CD8+ cells

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
1KL9 X-ray 1.9Å A=2-183.
1Q8K NMR - A=5-303.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Tat competes with 9079663
Pr55(Gag) co-localizes with 25229650
Tat induces phosphorylation of 19009018
Tat interacts with 10648795

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa03013 RNA transport - Homo sapiens (human)
hsa04141 Protein processing in endoplasmic reticulum - Homo sapiens (human)
hsa04210 Apoptosis - Homo sapiens (human)
hsa04932 Non-alcoholic fatty liver disease (NAFLD) - Homo sapiens (human)
hsa05160 Hepatitis C - Homo sapiens (human)
hsa05162 Measles - Homo sapiens (human)
hsa05164 Influenza A - Homo sapiens (human)
hsa05168 Herpes simplex infection - Homo sapiens (human)