Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0000907
UniProt IDQ96KQ7
Primary gene name(s)EHMT2
Synonym gene name(s)BAT8, C6orf30, G9A, KMT1C, NG36
Protein nameHistone-lysine N-methyltransferase EHMT2
Protein functionHistone methyltransferase that specifically mono- and dimethylates 'Lys-9' of histone H3, H3K9me1 and H3K9me2, respectively in euchromatin. H3K9me represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Also mediates monomethylation of 'Lys-56' of histone H3, H3K56me1 in G1 phase, leading to promote interaction between histone H3 and PCNA and regulating DNA replication. Also weakly methylates 'Lys-27' of histone H3, H3K27me. Also required for DNA methylation, the histone methyltransferase activity is not required for DNA methylation, suggesting that these 2 activities function independently. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. May also methylate histone H1. In addition to the histone methyltransferase activity, also methylates non-histone proteins: mediates dimethylation of 'Lys-373' of p53/TP53. Also methylates CDYL, WIZ, ACIN1, DNMT1, HDAC1, ERCC6, KLF12 and itself. {ECO:0000269|PubMed:11316813, ECO:0000269|PubMed:18438403, ECO:0000269|PubMed:20084102, ECO:0000269|PubMed:20118233, ECO:0000269|PubMed:22387026, ECO:0000269|PubMed:8457211}.
Subcellular locationNucleus {ECO:0000269|PubMed:11316813}. Chromosome {ECO:0000269|PubMed:11316813}. Note=Associates with euchromatic regions. Does not associate with heterochromatin.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q96KQ7
Gene Ontology
(Biological Process)
Complete annatation
cellular response to starvation [GO:0009267];
DNA methylation [GO:0006306];
histone lysine methylation [GO:0034968];
histone methylation [GO:0016571];
negative regulation of transcription from RNA polymerase II promoter [GO:0000122];
peptidyl-lysine dimethylation [GO:0018027];
regulation of DNA replication [GO:0006275];
regulation of signal transduction by p53 class mediator [GO:1901796]
Gene Ontology
(Molecular Function)
Complete annatation
C2H2 zinc finger domain binding [GO:0070742];
histone-lysine N-methyltransferase activity [GO:0018024];
histone methyltransferase activity, H3-K27 specific [GO:0046976];
histone methyltransferase activity, H3-K9 specific [GO:0046974];
p53 binding [GO:0002039];
promoter-specific chromatin binding [GO:1990841];
protein-lysine N-methyltransferase activity [GO:0016279];
zinc ion binding [GO:0008270]
Gene Ontology
(Cellular Component)
Complete annatation
nuclear chromatin [GO:0000790];
nucleoplasm [GO:0005654];
nucleus [GO:0005634]
Protein-protein interaction116123
Phylogenetic treeQ96KQ7
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.04759358957485540.8844563667492480.924447042971197
AZA vs. DISU0.2789215182967570.2736697853272810.846086444137247
AZA vs. IL7-0.112183920465470.560475838491590.999311006273513
AZA vs. SAHA0.06863217154316240.7785432747890580.941353350035522
DISU vs. CD30.2188191916984310.5509359490888610.669958736412189
DISU vs. IL7-0.4007052459151540.113237345678990.441593629160113
DISU vs. SAHA-0.2077482031018450.479624307694490.811332577063174
DMSO vs. AZA0.07583622235089880.6515399553814051
DMSO vs. CD30.01530022314985910.961892028973530.973458512460448
DMSO vs. DISU-0.2052963829414490.4021474051477780.86564225047522
DMSO vs. IL7-0.1803975972157340.3166100199720820.797189703271907
DMSO vs. SAHA-0.01195472614726850.9595943288322430.99060990101294
HIV vs. Mock in Activation0.2523145437901330.6862292396380780.999983755607037
HIV vs. Mock in Latency0.04830833136013790.7701329459049250.999834320637052
IL7 vs. CD3-0.1544855380421590.6316873209557290.743106453505479
SAHA vs. CD3-0.002208468815279040.9950092837373650.996421813157033
SAHA vs. IL70.1781145620078410.4655602905997370.70059457127887
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.128762 0.489804
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.025 0.961 0.88 0.842 1.172
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
2O8J X-ray 1.8Å A/B/C/D=913-1193.
3DM1 X-ray 2.4Å B/D/F/H=179-190.
3K5K X-ray 1.7Å A/B=913-1193.
3RJW X-ray 2.5Å A/B=913-1193.
4NVQ X-ray 2.0Å A/B=913-1193.
5JHN X-ray 1.6Å A/B=916-1189.
5JIN X-ray 1.8Å A/B=916-1189.
5JIY X-ray 1.4Å A/B=916-1189.
5JJ0 X-ray 1.7Å A/B=916-1189.
5T0K X-ray 1.7Å A/B=913-1193.
5T0M X-ray 1.9Å A/B=913-1193.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Tat inhibited by 20335163

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa00310 Lysine degradation - Homo sapiens (human)
hsa04211 Longevity regulating pathway - Homo sapiens (human)