Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0000893
UniProt IDA0AVK6
Primary gene name(s)E2F8
Synonym gene name(s)unknown
Protein nameTranscription factor E2F8
Protein functionAtypical E2F transcription factor that participates in various processes such as angiogenesis and polyploidization of specialized cells. Mainly acts as a transcription repressor that binds DNA independently of DP proteins and specifically recognizes the E2 recognition site 5'-TTTC[CG]CGC-3'. Directly represses transcription of classical E2F transcription factors such as E2F1: component of a feedback loop in S phase by repressing the expression of E2F1, thereby preventing p53/TP53-dependent apoptosis. Plays a key role in polyploidization of cells in placenta and liver by regulating the endocycle, probably by repressing genes promoting cytokinesis and antagonizing action of classical E2F proteins, E2F1, E2F2 and/or E2F3. Required for placental development by promoting polyploidization of trophoblast giant cells. Acts as a promoter of sprouting angiogenesis, possibly by acting as a transcription activator: associates with HIF1A, recognizes and binds the VEGFA promoter, which is different from canonical E2 recognition site, and activates expression of the VEGFA gene. {ECO:0000269|PubMed:15897886, ECO:0000269|PubMed:16179649, ECO:0000269|PubMed:18202719, ECO:0000269|PubMed:22903062}.
Subcellular locationNucleus {ECO:0000269|PubMed:15897886}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: A0AVK6
Gene Ontology
(Biological Process)
Complete annatation
cell cycle comprising mitosis without cytokinesis [GO:0033301];
cell proliferation [GO:0008283];
chorionic trophoblast cell differentiation [GO:0060718];
hepatocyte differentiation [GO:0070365];
negative regulation of cytokinesis [GO:0032466];
negative regulation of transcription from RNA polymerase II promoter [GO:0000122];
placenta development [GO:0001890];
positive regulation of DNA endoreduplication [GO:0032877];
positive regulation of transcription from RNA polymerase II promoter [GO:0045944];
sprouting angiogenesis [GO:0002040];
transcription, DNA-templated [GO:0006351];
trophoblast giant cell differentiation [GO:0060707]
Gene Ontology
(Molecular Function)
Complete annatation
core promoter binding [GO:0001047];
RNA polymerase II core promoter proximal region sequence-specific DNA binding [GO:0000978];
transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding [GO:0001078];
transcription corepressor activity [GO:0003714];
transcription factor activity, sequence-specific DNA binding [GO:0003700]
Gene Ontology
(Cellular Component)
Complete annatation
nucleus [GO:0005634];
transcription factor complex [GO:0005667]
Protein-protein interaction122847
Phylogenetic treeA0AVK6
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD31.278039740516850.5240727532142340.63896008222334
AZA vs. DISU-0.7540852534224180.07664860118964270.590222266291186
AZA vs. IL70.2704066832701690.610301244357710.999311006273513
AZA vs. SAHA-0.6968515600626310.1113345539142110.425642145306662
DISU vs. CD3-2.052461567369430.2824423832272350.412232424938743
DISU vs. IL71.020042560102350.08805223088364310.392055100429897
DISU vs. SAHAunknownunknownunknown
DMSO vs. AZA0.003346977945188190.9917927035847831
DMSO vs. CD3-1.28531281735840.5235776812141120.629923723094982
DMSO vs. DISU0.7563888977005070.07625361275533160.515310288889169
DMSO vs. IL70.2734972565895380.6069426768302160.913686923628471
DMSO vs. SAHA-0.7083426415371460.104066525422970.381509755448694
HIV vs. Mock in Activation0.1454095594418740.9577092701172030.999983755607037
HIV vs. Mock in Latency0.5025760397889550.4773626594596360.999834320637052
IL7 vs. CD3-1.0040176599140.5900817523130650.708201469274213
SAHA vs. CD3-2.00877745798510.3019064720214930.41527295850346
SAHA vs. IL7-0.9743185596839460.107290198486870.299467833718361
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change -1.732474372
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
2.7 0.005091791 1.6 0.089825151 1.7 0.251875188
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.65723 0.000305467
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
219990_at 3.22 No upregulated in CD8+ cells

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
4YO2 X-ray 3.0Å A=110-341.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
not found