Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0000891
UniProt IDQ16254
Primary gene name(s)E2F4
Synonym gene name(s)unknown
Protein nameTranscription factor E2F4
Protein functionTranscription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F4 binds with high affinity to RBL1 and RBL2. In some instances can also bind RB1. Specifically required for multiciliate cell differentiation: together with MCIDAS and E2F5, binds and activate genes required for centriole biogenesis. {ECO:0000250|UniProtKB:Q6DE14, ECO:0000269|PubMed:7958924, ECO:0000269|PubMed:7958925}.
Subcellular locationNucleus.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q16254
Gene Ontology
(Biological Process)
Complete annatation
animal organ morphogenesis [GO:0009887];
blood circulation [GO:0008015];
cell volume homeostasis [GO:0006884];
centriole assembly [GO:0098534];
DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest [GO:0006977];
epithelial cell development [GO:0002064];
motile cilium assembly [GO:0044458];
multi-ciliated epithelial cell differentiation [GO:1903251];
positive regulation of transcription from RNA polymerase II promoter [GO:0045944];
regulation of cell proliferation [GO:0042127];
regulation of transcription involved in G1/S transition of mitotic cell cycle [GO:0000083]
Gene Ontology
(Molecular Function)
Complete annatation
DNA binding [GO:0003677];
protein domain specific binding [GO:0019904];
RNA polymerase II core promoter proximal region sequence-specific DNA binding [GO:0000978];
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding [GO:0001077];
transcription factor activity, sequence-specific DNA binding [GO:0003700];
transcription factor binding [GO:0008134]
Gene Ontology
(Cellular Component)
Complete annatation
nuclear chromatin [GO:0000790];
nucleoplasm [GO:0005654];
transcription factor complex [GO:0005667]
Protein-protein interaction108206
Phylogenetic treeQ16254
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.7217304485728660.02901017741377550.0632949325391466
AZA vs. DISU0.01498540036391960.9527017525667930.996649735504266
AZA vs. IL70.05809316936282670.7622869857286480.999311006273513
AZA vs. SAHA-0.2453043984664920.3150349307344140.687042324347593
DISU vs. CD3-0.7192683845834870.04953288725359480.10710927305418
DISU vs. IL70.03377368934930940.8932088414751990.98042516255944
DISU vs. SAHA-0.2576627401357620.3770003502255520.74567834909247
DMSO vs. AZA0.003469547751960140.9834606608469121
DMSO vs. CD3-0.7318882112647650.02350944842356570.0512472638548716
DMSO vs. DISU-0.01390678484858650.9544894399524540.993828264951078
DMSO vs. IL70.06233582543840710.7285451666496680.94524644007606
DMSO vs. SAHA-0.253225061592180.2833436291024570.63375954796722
HIV vs. Mock in Activation0.06341274486904310.9188010355517420.999983755607037
HIV vs. Mock in Latency-0.03601379836450190.8274167800447660.999834320637052
IL7 vs. CD3-0.6576727010893930.04271233729680750.0962912144891982
SAHA vs. CD3-0.9900601237022260.005515548650222080.0155301107172688
SAHA vs. IL7-0.3056383408787460.2102489220689630.445716798557456
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.0690002 0.69046
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
1CF7 X-ray 2.6Å A=11-86.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Tat recruits 12055184

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04110 Cell cycle - Homo sapiens (human)
hsa04350 TGF-beta signaling pathway - Homo sapiens (human)