Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0000886
UniProt IDP63172
Primary gene name(s)DYNLT1
Synonym gene name(s)TCTEL1, TCTEX-1, TCTEX1
Protein nameDynein light chain Tctex-type 1
Protein functionActs as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. Binds to transport cargos and is involved in apical cargo transport such as rhodopsin-bearing vesicles in polarized epithelia. Is involved in intracellular targeting of D-type retrovirus gag polyproteins to the cytoplasmic assembly site. May also be a accessory component of axonemal dynein. {ECO:0000269|PubMed:18647839}.; FUNCTION: Plays a role in neuronal morphogenesis; the function is independent of cytoplasmic dynein and seems to be coupled to regulation of the actin cytoskeleton by enhancing Rac1 activity. The function in neurogenesis may be regulated by association with a G-protein beta-gamma dimer. May function as a receptor-independent activator of heterotrimeric G-protein signaling; the activation appears to be independent of a nucleotide exchange. Plays a role in regulating neurogenesis; inhibits the genesis of neurons from precursor cells during cortical development presumably by antagonizing ARHGEF2. Involved in the regulation of mitotic spindle orientation, By similarity. {ECO:0000250}.
Subcellular locationGolgi apparatus {ECO:0000250}. Cytoplasm {ECO:0000250}. Cytoplasm, cytoskeleton, spindle {ECO:0000250}. Note=Localizes to mitotic spindles. {ECO:0000250}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P63172
Gene Ontology
(Biological Process)
Complete annatation
cell division [GO:0051301];
establishment of mitotic spindle orientation [GO:0000132];
intracellular transport of viral protein in host cell [GO:0019060];
microtubule-dependent intracellular transport of viral material towards nucleus [GO:0075521];
mitotic nuclear division [GO:0007067];
negative regulation of neurogenesis [GO:0050768];
neuron projection morphogenesis [GO:0048812];
regulation of cytoskeleton organization [GO:0051493];
regulation of G-protein coupled receptor protein signaling pathway [GO:0008277];
regulation of GTPase activity [GO:0043087];
viral entry into host cell [GO:0046718]
Gene Ontology
(Molecular Function)
Complete annatation
identical protein binding [GO:0042802];
motor activity [GO:0003774]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasmic dynein complex [GO:0005868];
cytoplasmic microtubule [GO:0005881];
Golgi apparatus [GO:0005794];
host cell [GO:0043657];
secretory vesicle [GO:0099503];
spindle [GO:0005819]
Protein-protein interaction112853
Phylogenetic treeP63172
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.4827630070601650.1427834494332080.23338381410447
AZA vs. DISU0.07851254449481630.7571655719800280.976021571236163
AZA vs. IL7-0.09252210516491980.633438153334050.999311006273513
AZA vs. SAHA0.1369599914826590.5780997918215250.860159536266674
DISU vs. CD3-0.4167541415215780.2519223803702030.379051831677446
DISU vs. IL7-0.1800841467854190.4766229170300630.812149934918977
DISU vs. SAHA0.0595377159118870.8393792757128550.958240859876863
DMSO vs. AZA0.02553303419041090.8802952283523441
DMSO vs. CD3-0.4695830035024870.145355232774460.231184183039194
DMSO vs. DISU-0.05507490523607280.8221990281144980.980295078677667
DMSO vs. IL7-0.1108271785732150.541384316678980.896696737481162
DMSO vs. SAHA0.1047342204314660.6589346259102380.892524396635398
HIV vs. Mock in Activation-0.3963384526575630.5248376784693710.999983755607037
HIV vs. Mock in Latency-0.0313684036619720.8501951981010220.999834320637052
IL7 vs. CD3-0.5669200009346550.08001197334845990.159273702361508
SAHA vs. CD3-0.3706815606017210.3022329283636620.415570276500035
SAHA vs. IL70.2257840296925180.3577394727593590.608473847802264
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
0.4862 0.03183

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.413605 0.00457947
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.112 1.06 1.164 1.258 1.118
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
5JPW NMR - A/B=1-113.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
not found