Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0000876
UniProt IDQ9BY84
Primary gene name(s)DUSP16
Synonym gene name(s)KIAA1700, MKP7
Protein nameDual specificity protein phosphatase 16
Protein functionDual specificity protein phosphatase involved in the inactivation of MAP kinases. Dephosphorylates MAPK10 bound to ARRB2. {ECO:0000269|PubMed:11489891, ECO:0000269|PubMed:15888437}.
Subcellular locationCytoplasm. Nucleus. Cytoplasmic vesicle. Note=After dissociation upon AGTR stimulation, re-associates with ARRB2 on endocytic vesicles.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q9BY84
Gene Ontology
(Biological Process)
Complete annatation
dephosphorylation [GO:0016311];
inactivation of MAPK activity [GO:0000188];
MAPK export from nucleus [GO:0045204];
MAPK phosphatase export from nucleus, leptomycin B sensitive [GO:0045209]
Gene Ontology
(Molecular Function)
Complete annatation
MAP kinase tyrosine/serine/threonine phosphatase activity [GO:0017017];
phosphoprotein phosphatase activity [GO:0004721];
protein tyrosine phosphatase activity [GO:0004725]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasm [GO:0005737];
cytoplasmic, membrane-bounded vesicle [GO:0016023];
cytosol [GO:0005829];
nucleoplasm [GO:0005654];
nucleus [GO:0005634]
Protein-protein interaction123321
Phylogenetic treeQ9BY84
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.9462416237204150.00420424935156960.0125577578729404
AZA vs. DISU0.05778471853581310.8199858631214480.984374783402915
AZA vs. IL7-0.4128379793915130.03263273657536360.563712690119688
AZA vs. SAHA-0.4062242058555380.09786121405378930.395379238983334
DISU vs. CD30.9925000305602950.006731029644435390.0208515291533537
DISU vs. IL7-0.4804203573385020.05744998345399320.315618987087809
DISU vs. SAHA-0.4625175833764520.1132312068883520.432659876925139
DMSO vs. AZA-0.06993330324581530.677271905520241
DMSO vs. CD30.8650496994365240.007261577666143550.0189102277511586
DMSO vs. DISU-0.1295506580627520.5964560501042940.937098979571498
DMSO vs. IL7-0.3357916545447550.06295353724062590.481005866144164
DMSO vs. SAHA-0.3425254519479540.1479758110283410.46328121466226
HIV vs. Mock in Activation0.4058620073151650.5157988014837730.999983755607037
HIV vs. Mock in Latency0.2986600562650830.0713757175852960.999834320637052
IL7 vs. CD30.5395479486442130.09416881432724290.180914463593726
SAHA vs. CD30.5160155832807910.1456355369144470.235435265104131
SAHA vs. IL70.003344359065930890.9890668287903850.995763650076747
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -1.28573 0.000305467
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
2VSW X-ray 2.2Å A/B=5-150.
3TG3 X-ray 2.6Å A/B/C/D=5-138.
4YR8 X-ray 2.4Å B/D/G/H=156-301.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
HIV-1 virus replication enhanced by expression of human gene 18854154

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04010 MAPK signaling pathway - Homo sapiens (human)