Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0000854
UniProt IDQ14185
Primary gene name(s)DOCK1
Synonym gene name(s)unknown
Protein nameDedicator of cytokinesis protein 1
Protein functionInvolved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Along with DOCK1, mediates CRK/CRKL regulation of epithelial and endothelial cell spreading and migration on type IV collagen, PubMed:19004829. Functions as a guanine nucleotide exchange factor, GEF, which activates Rac Rho small GTPases by exchanging bound GDP for free GTP. Its GEF activity may be enhanced by ELMO1, PubMed:8657152. {ECO:0000269|PubMed:19004829, ECO:0000269|PubMed:8657152}.
Subcellular locationCytoplasm {ECO:0000305}. Membrane {ECO:0000305}. Note=Recruited to membranes via its interaction with phosphatidylinositol 3,4,5-trisphosphate. {ECO:0000305}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q14185
Gene Ontology
(Biological Process)
Complete annatation
apoptotic process [GO:0006915];
blood coagulation [GO:0007596];
cell migration [GO:0016477];
cytoskeleton organization [GO:0007010];
Fc-gamma receptor signaling pathway involved in phagocytosis [GO:0038096];
integrin-mediated signaling pathway [GO:0007229];
phagocytosis, engulfment [GO:0006911];
positive regulation of epithelial cell migration [GO:0010634];
positive regulation of substrate adhesion-dependent cell spreading [GO:1900026];
signal transduction [GO:0007165];
small GTPase mediated signal transduction [GO:0007264];
vascular endothelial growth factor receptor signaling pathway [GO:0048010]
Gene Ontology
(Molecular Function)
Complete annatation
GTPase activator activity [GO:0005096];
Rho guanyl-nucleotide exchange factor activity [GO:0005089]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasm [GO:0005737];
cytosol [GO:0005829];
guanyl-nucleotide exchange factor complex [GO:0032045];
membrane [GO:0016020];
nucleoplasm [GO:0005654];
nucleus [GO:0005634]
Protein-protein interaction108128
Phylogenetic treeQ14185
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-1.093098748289010.09582925324409410.169902188553698
AZA vs. DISU-0.2945228371654440.6876628607268470.968313969505284
AZA vs. IL7-0.6114313650395360.3374859176773880.999311006273513
AZA vs. SAHA-0.6275577860720590.4185795316258710.773260458856321
DISU vs. CD30.7833557918109060.3376484424783630.470964432489946
DISU vs. IL7-0.3247662680984220.6611829050064920.904437359612644
DISU vs. SAHA-0.33405304131520.6937162685961480.909397757924307
DMSO vs. AZA-0.02837774504036370.9648678850123431
DMSO vs. CD31.042409690719680.1223137186877440.200878248340877
DMSO vs. DISU0.2623132915498450.7242298204969990.964232122527588
DMSO vs. IL7-0.5744630524045450.3790053410554150.832872524258073
DMSO vs. SAHA-0.6055695344252470.4404460105589370.773504721891803
HIV vs. Mock in Activation-0.6923540958815050.4839409779364360.999983755607037
HIV vs. Mock in Latency0.1029860734802160.8582900724000370.999834320637052
IL7 vs. CD30.4884611758955780.4705083402147630.60285380925341
SAHA vs. CD30.4408472530020830.6123076257180060.708062915866357
SAHA vs. IL7-0.0211113901568950.9783824016440880.992271837609028
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change -1.214188463
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.548756 0.000305467
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
3L4C X-ray 2.3Å A/B=422-619.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04510 Focal adhesion - Homo sapiens (human)
hsa04810 Regulation of actin cytoskeleton - Homo sapiens (human)
hsa05100 Bacterial invasion of epithelial cells - Homo sapiens (human)
hsa05131 Shigellosis - Homo sapiens (human)