Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0000852
UniProt IDQ14183
Primary gene name(s)DOC2A
Synonym gene name(s)unknown
Protein nameDouble C2-like domain-containing protein alpha
Protein functionCalcium sensor which most probably regulates fusion of vesicles with membranes. Binds calcium and phospholipids. May be involved in calcium dependent neurotransmitter release through the interaction with UNC13A. May be involved in calcium-dependent spontaneous release of neurotransmitter in absence of action potentials in neuronal cells. Regulates Ca(2+-dependent secretory lysosome exocytosis in mast cells. {ECO:0000269|PubMed:18354201, ECO:0000269|PubMed:9736751, ECO:0000269|PubMed:9804756}.
Subcellular locationLysosome {ECO:0000250}. Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane {ECO:0000305};
Peripheral membrane protein {ECO:0000305}. Cell junction, synapse, synaptosome. Note=Colocalizes to synaptic vesicles.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q14183
Gene Ontology
(Biological Process)
Complete annatation
calcium ion-regulated exocytosis of neurotransmitter [GO:0048791];
chemical synaptic transmission [GO:0007268];
nervous system development [GO:0007399];
regulation of calcium ion-dependent exocytosis [GO:0017158];
vesicle fusion [GO:0006906]
Gene Ontology
(Molecular Function)
Complete annatation
calcium-dependent phospholipid binding [GO:0005544];
calcium ion binding [GO:0005509];
clathrin binding [GO:0030276];
syntaxin binding [GO:0019905]
Gene Ontology
(Cellular Component)
Complete annatation
cell junction [GO:0030054];
cytosol [GO:0005829];
lysosome [GO:0005764];
neuron projection [GO:0043005];
plasma membrane [GO:0005886];
synaptic vesicle membrane [GO:0030672]
Protein-protein interaction114026
Phylogenetic treeQ14183
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.1937508386307490.572808254550740.681251641504055
AZA vs. DISU-0.004429815746657730.9871953688349750.998932793566471
AZA vs. IL7-0.1318819146072720.5657971695699570.999311006273513
AZA vs. SAHA-0.148493140727390.5838398583136980.86190861711434
DISU vs. CD3-0.2101792850637550.5769745482514440.692103902014634
DISU vs. IL7-0.1396419339547440.6106679992564660.880844111640685
DISU vs. SAHA-0.1417934862396060.64937458109750.891714689203312
DMSO vs. AZA-0.05328488283751910.8105692023450711
DMSO vs. CD3-0.2657817309099710.4278578702335350.541926922403582
DMSO vs. DISU-0.05259427702697320.8432876275465420.982583769983649
DMSO vs. IL7-0.06984880443461030.741278028698740.948337432944511
DMSO vs. SAHA-0.09735408101635320.709588127130090.912318944205561
HIV vs. Mock in Activation0.103956666528610.8716116408880790.999983755607037
HIV vs. Mock in Latency0.04378091298607240.8159455136388720.999834320637052
IL7 vs. CD3-0.3223941862657690.3390234390432960.476643997453559
SAHA vs. CD3-0.3662665654142850.3209334355669530.435953386875246
SAHA vs. IL7-0.01682096990847670.9502380675553560.981088262351331
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.239243 0.924914
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
4MJJ X-ray 2.0Å A=81-217.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
not found