Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0000845
UniProt IDO00115
Primary gene name(s)DNASE2
Synonym gene name(s)DNASE2A, DNL2
Protein nameDeoxyribonuclease-2-alpha
Protein functionHydrolyzes DNA under acidic conditions with a preference for double-stranded DNA. Plays a major role in the degradation of nuclear DNA in cellular apoptosis during development. Necessary for proper fetal development and for definitive erythropoiesis in fetal liver, where it degrades nuclear DNA expelled from erythroid precursor cells.
Subcellular locationLysosome.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: O00115
Gene Ontology
(Biological Process)
Complete annatation
apoptotic DNA fragmentation [GO:0006309];
DNA metabolic process [GO:0006259];
erythrocyte differentiation [GO:0030218]
Gene Ontology
(Molecular Function)
Complete annatation
deoxyribonuclease II activity [GO:0004531];
DNA binding [GO:0003677]
Gene Ontology
(Cellular Component)
Complete annatation
extracellular exosome [GO:0070062];
lysosome [GO:0005764]
Protein-protein interaction108116
Phylogenetic treeO00115
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-1.010037210060690.00267106403546680.0085166653570035
AZA vs. DISU0.1724160264754560.497423434712370.934754900374469
AZA vs. IL7-0.2021609035060790.297869731514460.999311006273513
AZA vs. SAHA-0.5223258956715330.03427446110545260.215003423513771
DISU vs. CD31.170639580082950.001573534551547850.00609600529119465
DISU vs. IL7-0.3845465961979980.1286455855964840.469799467748787
DISU vs. SAHA-0.692205263224540.01842161952849250.154068938908941
DMSO vs. AZA-0.01705379178262660.9198058290624921
DMSO vs. CD30.9809901371058880.002735999145657480.00820418533625196
DMSO vs. DISU-0.1915367542155160.4342354413787650.881931010994338
DMSO vs. IL7-0.1777498938968050.3276148537819790.802824414281686
DMSO vs. SAHA-0.5100331356233360.03220591739183460.19338563034238
HIV vs. Mock in Activation0.09813587136809250.8752382120202910.999983755607037
HIV vs. Mock in Latency0.1993652709616790.2316950897174820.999834320637052
IL7 vs. CD30.8133478865174090.01275007120668860.0363224009258017
SAHA vs. CD30.4650507300814530.1924422965012560.292999131973522
SAHA vs. IL7-0.3220882060685680.1891767907148670.419382488640523
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.0915008 0.589848
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.06 0.958 0.919 1.091 1.075
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Envelope surface glycoprotein gp120 upregulates 24162774

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04142 Lysosome - Homo sapiens (human)