Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0000836
UniProt IDO75190
Primary gene name(s)DNAJB6
Synonym gene name(s)HSJ2, MRJ, MSJ1
Protein nameDnaJ homolog subfamily B member 6
Protein functionPlays an indispensable role in the organization of KRT8/KRT18 filaments. Acts as an endogenous molecular chaperone for neuronal proteins including huntingtin. Suppresses aggregation and toxicity of polyglutamine-containing, aggregation-prone proteins. Isoform B but not isoform A inhibits huntingtin aggregation. Has a stimulatory effect on the ATPase activity of HSP70 in a dose-dependent and time-dependent manner and hence acts as a co-chaperone of HSP70. Also reduces cellular toxicity and caspase-3 activity. {ECO:0000269|PubMed:10954706, ECO:0000269|PubMed:11896048, ECO:0000269|PubMed:20159555, ECO:0000269|PubMed:22366786}.
Subcellular locationCytoplasm, perinuclear region {ECO:0000269|PubMed:10954706}. Nucleus {ECO:0000269|PubMed:10954706}. Cytoplasm, myofibril, sarcomere, Z line {ECO:0000269|PubMed:22366786}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: O75190
Gene Ontology
(Biological Process)
Complete annatation
actin cytoskeleton organization [GO:0030036];
chorio-allantoic fusion [GO:0060710];
chorion development [GO:0060717];
extracellular matrix organization [GO:0030198];
intermediate filament organization [GO:0045109];
negative regulation of cysteine-type endopeptidase activity involved in apoptotic process [GO:0043154];
negative regulation of inclusion body assembly [GO:0090084];
negative regulation of transcription, DNA-templated [GO:0045892];
protein folding [GO:0006457];
protein localization to nucleus [GO:0034504];
regulation of cellular response to heat [GO:1900034];
regulation of protein localization [GO:0032880];
syncytiotrophoblast cell differentiation involved in labyrinthine layer development [GO:0060715]
Gene Ontology
(Molecular Function)
Complete annatation
ATPase activator activity [GO:0001671];
chaperone binding [GO:0051087];
DNA binding [GO:0003677];
heat shock protein binding [GO:0031072];
unfolded protein binding [GO:0051082]
Gene Ontology
(Cellular Component)
Complete annatation
cytosol [GO:0005829];
membrane [GO:0016020];
nucleoplasm [GO:0005654];
nucleus [GO:0005634];
perinuclear region of cytoplasm [GO:0048471];
Z disc [GO:0030018]
Protein-protein interaction115360
Phylogenetic treeO75190
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD31.428692728152961.8489084908424e-050.000107221041839079
AZA vs. DISU0.5973178542583110.01873201843150320.325422257378601
AZA vs. IL70.1024060129902250.5946350308442220.999311006273513
AZA vs. SAHA0.06880044074031590.7782558652912120.941353350035522
DISU vs. CD3-0.8448997310078940.02103432084742430.0536590544007571
DISU vs. IL7-0.5034244351909930.04661688846469370.28536724079039
DISU vs. SAHA-0.5273565909588480.07260198386013980.340655198865557
DMSO vs. AZA-0.0609570406632810.7164622213201071
DMSO vs. CD3-1.501740752789914.20308687720805e-062.62954750021343e-05
DMSO vs. DISU-0.6604541338966740.007104416168942750.181229062518652
DMSO vs. IL70.1707555669162130.3429055374703150.812783172541666
DMSO vs. SAHA0.1224039597504960.6041332408064310.86649389711633
HIV vs. Mock in Activation-0.008080357815999860.9896243574594520.999983755607037
HIV vs. Mock in Latency-0.1279795323708520.4386082397689580.999834320637052
IL7 vs. CD3-1.317471763412385.39131165303575e-050.000339165788044514
SAHA vs. CD3-1.385445576229660.0001152784394620190.000545788904396049
SAHA vs. IL7-0.03768820119007790.8770993318487790.950769116085754
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change -0.930508388
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.779687 0.637726
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.649 0.621 0.534 0.559 0.655
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Rev inhibited by 24047968
capsid inhibited by 24047968
Vpr facilitated by 26137520
HIV-1 virus replication enhanced by expression of human gene 18976975

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
not found