Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0000834
UniProt IDP25686
Primary gene name(s)DNAJB2
Synonym gene name(s)HSJ1, HSPF3
Protein nameDnaJ homolog subfamily B member 2
Protein functionFunctions as a co-chaperone, regulating the substrate binding and activating the ATPase activity of chaperones of the HSP70/heat shock protein 70 family, PubMed:7957263, PubMed:22219199. In parallel, also contributes to the ubiquitin-dependent proteasomal degradation of misfolded proteins, PubMed:15936278, PubMed:21625540. Thereby, may regulate the aggregation and promote the functional recovery of misfolded proteins like HTT, MC4R, PARK2, RHO and SOD1 and be crucial for many biological processes, PubMed:12754272, PubMed:20889486, PubMed:21719532, PubMed:22396390, PubMed:24023695. Isoform 1 which is localized to the endoplasmic reticulum membranes may specifically function in ER-associated protein degradation of misfolded proteins, PubMed:15936278. {ECO:0000269|PubMed:12754272, ECO:0000269|PubMed:15936278, ECO:0000269|PubMed:20889486, ECO:0000269|PubMed:21625540, ECO:0000269|PubMed:21719532, ECO:0000269|PubMed:22219199, ECO:0000269|PubMed:22396390, ECO:0000269|PubMed:24023695, ECO:0000269|PubMed:7957263}.
Subcellular locationIsoform 2: Cytoplasm {ECO:0000269|PubMed:12754272, ECO:0000269|PubMed:21625540}. Nucleus {ECO:0000269|PubMed:12754272}.;
SUBCELLULAR LOCATION: Isoform 1: Endoplasmic reticulum membrane {ECO:0000269|PubMed:12754272};
Lipid-anchor {ECO:0000269|PubMed:12754272};
Cytoplasmic side {ECO:0000269|PubMed:12754272}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P25686
Gene Ontology
(Biological Process)
Complete annatation
chaperone-mediated protein folding [GO:0061077];
ER-associated ubiquitin-dependent protein catabolic process [GO:0030433];
negative regulation of cell growth [GO:0030308];
negative regulation of cell proliferation [GO:0008285];
negative regulation of inclusion body assembly [GO:0090084];
negative regulation of protein binding [GO:0032091];
negative regulation of protein deubiquitination [GO:0090086];
positive regulation of ATPase activity [GO:0032781];
positive regulation of proteasomal ubiquitin-dependent protein catabolic process [GO:0032436];
positive regulation of protein ubiquitination [GO:0031398];
proteasome-mediated ubiquitin-dependent protein catabolic process [GO:0043161];
protein refolding [GO:0042026];
regulation of chaperone-mediated protein folding [GO:1903644];
regulation of protein localization [GO:0032880];
regulation of protein ubiquitination [GO:0031396];
response to unfolded protein [GO:0006986]
Gene Ontology
(Molecular Function)
Complete annatation
chaperone binding [GO:0051087];
Hsp70 protein binding [GO:0030544];
polyubiquitin binding [GO:0031593];
proteasome binding [GO:0070628];
ubiquitin protein ligase binding [GO:0031625];
unfolded protein binding [GO:0051082]
Gene Ontology
(Cellular Component)
Complete annatation
cytosol [GO:0005829];
inclusion body [GO:0016234];
nucleus [GO:0005634]
Protein-protein interaction109533
Phylogenetic treeP25686
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.7553618192526880.02185855703600480.0500231053213885
AZA vs. DISU0.3195615143083620.2072391139661280.796980014175012
AZA vs. IL7-0.3829823687606320.04776121122650490.644768550316037
AZA vs. SAHA-0.04653779022754520.8490104417869070.964408468086072
DISU vs. CD31.06214199975380.00374438643271680.0127641069847971
DISU vs. IL7-0.7122547971882420.00501885652113110.0751333287547679
DISU vs. SAHA-0.3633707414920650.2130031044276830.587473442131041
DMSO vs. AZA-0.04409408150548610.7933215070182751
DMSO vs. CD30.6999121003874860.02946255323671910.0618956023582726
DMSO vs. DISU-0.365547102590140.1349208250875440.625617487405936
DMSO vs. IL7-0.3314813842668230.0668736086770910.493209222508389
DMSO vs. SAHA-0.007766962495365160.9737760339593010.993688036278735
HIV vs. Mock in Activation-0.04475293355432090.9429873815642870.999983755607037
HIV vs. Mock in Latency0.08058546961115470.6263039352055720.999834320637052
IL7 vs. CD30.3779494248767010.2407833057743520.370679886419856
SAHA vs. CD30.6856288461866830.05355295389237740.104906150417019
SAHA vs. IL70.3342329085264430.1722216882079990.397240288747274
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.227572 0.36979
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.802 1.16 0.769 0.908 0.802
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
2LGW NMR - A=1-91.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Vpr downregulates 14751250
Vif interacts with 22190034

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04141 Protein processing in endoplasmic reticulum - Homo sapiens (human)