Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0000833
UniProt IDP25685
Primary gene name(s)DNAJB1
Synonym gene name(s)DNAJ1, HDJ1, HSPF1
Protein nameDnaJ homolog subfamily B member 1
Protein functionInteracts with HSP70 and can stimulate its ATPase activity. Stimulates the association between HSC70 and HIP.
Subcellular locationCytoplasm {ECO:0000269|PubMed:1586970}. Nucleus {ECO:0000269|PubMed:1586970}. Nucleus, nucleolus {ECO:0000269|PubMed:1586970}. Note=Translocates rapidly from the cytoplasm to the nucleus, and especially to the nucleoli, upon heat shock.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P25685
Gene Ontology
(Biological Process)
Complete annatation
chaperone cofactor-dependent protein refolding [GO:0070389];
chaperone mediated protein folding requiring cofactor [GO:0051085];
negative regulation of inclusion body assembly [GO:0090084];
positive regulation of ATPase activity [GO:0032781];
regulation of cellular response to heat [GO:1900034];
response to unfolded protein [GO:0006986]
Gene Ontology
(Molecular Function)
Complete annatation
ATPase activator activity [GO:0001671];
ATPase binding [GO:0051117];
chaperone binding [GO:0051087];
Hsp70 protein binding [GO:0030544];
unfolded protein binding [GO:0051082]
Gene Ontology
(Cellular Component)
Complete annatation
cytosol [GO:0005829];
extracellular exosome [GO:0070062];
nucleolus [GO:0005730];
nucleoplasm [GO:0005654];
nucleus [GO:0005634]
Protein-protein interaction109569
Phylogenetic treeP25685
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.1097313223559360.7383285435083360.817340272328876
AZA vs. DISU0.1683064763947850.5059179489264180.936713532722094
AZA vs. IL7-0.140836848072820.4644496290829380.999311006273513
AZA vs. SAHA-0.06992895346356980.7749741838461180.941106972310995
DISU vs. CD30.04587962528553540.8998442415070170.934692123516867
DISU vs. IL7-0.3186053932303740.2065685166368010.585243829162515
DISU vs. SAHA-0.2364060411489130.4193481178828220.773371938890358
DMSO vs. AZA-0.003012063192983730.985677129500671
DMSO vs. CD3-0.1257203380477670.6951270004553930.776112670411361
DMSO vs. DISU-0.173704168356890.4766014387697730.899259256574493
DMSO vs. IL7-0.1303342961882860.4691738114825770.877802004454825
DMSO vs. SAHA-0.07267342419668320.7585177568898830.930297284308295
HIV vs. Mock in Activation0.05830382269959760.9254549511886340.999983755607037
HIV vs. Mock in Latency-0.2897862452931920.1396337282809610.999834320637052
IL7 vs. CD3-0.2438652397976030.4492449539538470.583244547023801
SAHA vs. CD3-0.2039914906560310.5634651988309920.666046007246298
SAHA vs. IL70.06804885497151620.7804172851416150.903613129937757
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.672166 0.000305467
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.985 0.899 0.854 0.795 0.774
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
200664_s_at 1.53 No downregulated in CD8+ cells

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
1HDJ NMR - A=1-76.
2QLD X-ray 2.7Å A=158-340.
3AGX X-ray 1.8Å A/B=161-340.
3AGY X-ray 1.8Å A/B=161-340.
3AGZ X-ray 2.5Å A/B=151-340.
4WB7 X-ray 1.9Å A/B=2-70.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Nef interacts with 16179353
Tat upregulates 25472883
capsid inhibited by 24047968
HIV-1 virus replication enhanced by expression of human gene 18187620
Nef upregulates 16179353

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04141 Protein processing in endoplasmic reticulum - Homo sapiens (human)
hsa05164 Influenza A - Homo sapiens (human)