Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0000832
UniProt IDQ96EY1
Primary gene name(s)DNAJA3
Synonym gene name(s)HCA57, TID1
Protein nameDnaJ homolog subfamily A member 3, mitochondrial
Protein functionModulates apoptotic signal transduction or effector structures within the mitochondrial matrix. Affect cytochrome C release from the mitochondria and caspase 3 activation, but not caspase 8 activation. Isoform 1 increases apoptosis triggered by both TNF and the DNA-damaging agent mytomycin C; in sharp contrast, isoform 2 suppresses apoptosis. Can modulate IFN-gamma-mediated transcriptional activity. Isoform 2 may play a role in neuromuscular junction development as an effector of the MUSK signaling pathway.
Subcellular locationMitochondrion matrix. Cytoplasm, cytosol {ECO:0000250}. Cell junction, synapse, postsynaptic cell membrane {ECO:0000250};
Peripheral membrane protein {ECO:0000250}. Note=Recruited to the postsynaptic cell membrane of the neuromuscular junction through interaction with MUSK. {ECO:0000250}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q96EY1
Gene Ontology
(Biological Process)
Complete annatation
activation-induced cell death of T cells [GO:0006924];
activation of cysteine-type endopeptidase activity involved in apoptotic process [GO:0006919];
cell aging [GO:0007569];
mitochondrial DNA replication [GO:0006264];
mitochondrion organization [GO:0007005];
negative regulation of apoptotic process [GO:0043066];
negative regulation of cell proliferation [GO:0008285];
negative regulation of cysteine-type endopeptidase activity involved in apoptotic process [GO:0043154];
negative regulation of I-kappaB kinase/NF-kappaB signaling [GO:0043124];
negative regulation of interferon-gamma-mediated signaling pathway [GO:0060336];
negative regulation of NF-kappaB transcription factor activity [GO:0032088];
negative regulation of programmed cell death [GO:0043069];
negative regulation of protein kinase activity [GO:0006469];
negative regulation of transcription from RNA polymerase II promoter [GO:0000122];
neuromuscular junction development [GO:0007528];
positive regulation of apoptotic process [GO:0043065];
positive regulation of protein ubiquitination [GO:0031398];
positive regulation of T cell proliferation [GO:0042102];
protein folding [GO:0006457];
protein stabilization [GO:0050821];
response to heat [GO:0009408];
response to interferon-gamma [GO:0034341];
skeletal muscle acetylcholine-gated channel clustering [GO:0071340];
small GTPase mediated signal transduction [GO:0007264];
T cell differentiation in thymus [GO:0033077]
Gene Ontology
(Molecular Function)
Complete annatation
ATP binding [GO:0005524];
GTPase regulator activity [GO:0030695];
interferon-gamma receptor binding [GO:0005133];
metal ion binding [GO:0046872];
NF-kappaB binding [GO:0051059];
protein kinase binding [GO:0019901];
transcription factor binding [GO:0008134]
Gene Ontology
(Cellular Component)
Complete annatation
cell junction [GO:0030054];
cytoplasm [GO:0005737];
cytosol [GO:0005829];
extrinsic component of plasma membrane [GO:0019897];
intracellular membrane-bounded organelle [GO:0043231];
mitochondrial matrix [GO:0005759];
mitochondrial nucleoid [GO:0042645];
mitochondrion [GO:0005739];
neuromuscular junction [GO:0031594];
nucleus [GO:0005634];
postsynaptic membrane [GO:0045211]
Protein-protein interaction114547
Phylogenetic treeQ96EY1
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.8504928848684040.009857080508356760.0257658545650283
AZA vs. DISU-0.1155708057790720.6480849465715040.960057478314726
AZA vs. IL70.1039589789383520.5895873645951660.999311006273513
AZA vs. SAHA-0.07436079126222720.7609247591946210.935886888638505
DISU vs. CD3-0.9788109748060660.007397704744557810.0225233288869853
DISU vs. IL70.2103201132395140.4042677596470590.765331718784304
DISU vs. SAHA0.04346484638739930.881546053858430.96945856056033
DMSO vs. AZA0.02435644157467020.8848407696870291
DMSO vs. CD3-0.8396493091445080.009054637568407190.0228872676120896
DMSO vs. DISU0.1375534743756570.5732763464067070.930932477407238
DMSO vs. IL70.08726549936522380.6282764500967970.91917755312917
DMSO vs. SAHA-0.103732885991870.660528625781840.892974558461198
HIV vs. Mock in Activation0.1585614997092640.7986868056387650.999983755607037
HIV vs. Mock in Latency-0.02673864228591530.8718081503454320.999834320637052
IL7 vs. CD3-0.7401457435767910.02181600698695040.0560669519717482
SAHA vs. CD3-0.948425673994210.007808416027827780.0208381139612829
SAHA vs. IL7-0.1807490010090980.4587175716186530.695644735873197
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.11041 0.50217
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.964 0.961 0.848 0.781 0.85
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
2CTT NMR - A=213-303.
2DN9 NMR - A=93-158.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa05203 Viral carcinogenesis - Homo sapiens (human)