Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0000830
UniProt IDP31689
Primary gene name(s)DNAJA1
Synonym gene name(s)DNAJ2, HDJ2, HSJ2, HSPF4
Protein nameDnaJ homolog subfamily A member 1
Protein functionCo-chaperone for HSPA8/Hsc70, PubMed:10816573. Stimulates ATP hydrolysis, but not the folding of unfolded proteins mediated by HSPA1A, in vitro, PubMed:24318877. Plays a role in protein transport into mitochondria via its role as co-chaperone. Functions as co-chaperone for HSPA1B and negatively regulates the translocation of BAX from the cytosol to mitochondria in response to cellular stress, thereby protecting cells against apoptosis, PubMed:14752510. Promotes apoptosis in response to cellular stress mediated by exposure to anisomycin or UV, PubMed:24512202. {ECO:0000269|PubMed:10816573, ECO:0000269|PubMed:14752510, ECO:0000269|PubMed:24318877, ECO:0000269|PubMed:24512202, ECO:0000269|PubMed:9192730}.
Subcellular locationMembrane {ECO:0000305|PubMed:10816573};
Lipid-anchor {ECO:0000305|PubMed:10816573}. Cytoplasm {ECO:0000269|PubMed:10816573}. Microsome {ECO:0000250}. Nucleus {ECO:0000269|PubMed:10816573}. Cytoplasm, perinuclear region {ECO:0000269|PubMed:10816573}. Mitochondrion {ECO:0000250}. Note=Primarily associated with microsomes. A minor proportion is associated with mitochondria, By similarity. Primarily cytoplasmic. A minor proportion is associated with nuclei. {ECO:0000250}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P31689
Gene Ontology
(Biological Process)
Complete annatation
androgen receptor signaling pathway [GO:0030521];
DNA damage response, detection of DNA damage [GO:0042769];
flagellated sperm motility [GO:0030317];
negative regulation of apoptotic process [GO:0043066];
negative regulation of establishment of protein localization to mitochondrion [GO:1903748];
negative regulation of JUN kinase activity [GO:0043508];
negative regulation of nitrosative stress-induced intrinsic apoptotic signaling pathway [GO:1905259];
negative regulation of protein ubiquitination [GO:0031397];
positive regulation of apoptotic process [GO:0043065];
protein folding [GO:0006457];
protein localization to mitochondrion [GO:0070585];
regulation of protein transport [GO:0051223];
response to heat [GO:0009408];
response to unfolded protein [GO:0006986];
spermatogenesis [GO:0007283];
toxin transport [GO:1901998]
Gene Ontology
(Molecular Function)
Complete annatation
ATP binding [GO:0005524];
C3HC4-type RING finger domain binding [GO:0055131];
chaperone binding [GO:0051087];
G-protein coupled receptor binding [GO:0001664];
Hsp70 protein binding [GO:0030544];
low-density lipoprotein particle receptor binding [GO:0050750];
metal ion binding [GO:0046872];
ubiquitin protein ligase binding [GO:0031625]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasmic side of endoplasmic reticulum membrane [GO:0098554];
cytosol [GO:0005829];
extracellular exosome [GO:0070062];
membrane [GO:0016020];
mitochondrion [GO:0005739];
nucleus [GO:0005634];
perinuclear region of cytoplasm [GO:0048471]
Protein-protein interaction109534
Phylogenetic treeP31689
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD32.347157250709948.01669841621333e-121.6758346536639e-10
AZA vs. DISU0.5115947070294510.07075421389576730.577762199493389
AZA vs. IL70.5753963625413940.01185573086930040.358895163472903
AZA vs. SAHA-0.1626816705539930.5050274469992250.82403151499238
DISU vs. CD3-1.848947150987047.98244667254977e-077.92509680077746e-06
DISU vs. IL70.05517678965478230.857185368498260.972095797044404
DISU vs. SAHA-0.6734567362031910.02671534490902130.193410298048169
DMSO vs. AZA-0.09507766763503890.5695476888625831
DMSO vs. CD3-2.453517421366683.10640402290119e-137.34662113108421e-12
DMSO vs. DISU-0.6087956678101620.02054905729928960.294647282112554
DMSO vs. IL70.6778501045651070.0008520394463035210.0506573826877345
DMSO vs. SAHA-0.0754033982834510.7490459703369840.927100230037811
HIV vs. Mock in Activation-0.100077535920060.8720568452745610.999983755607037
HIV vs. Mock in Latency-0.2741164119910730.09687779509222160.999834320637052
IL7 vs. CD3-1.762543462856449.24049715633757e-081.14259240063557e-06
SAHA vs. CD3-2.53539518917411.13783427124758e-112.89159569438467e-10
SAHA vs. IL7-0.7419116097046310.003507651801531790.0299063027467704
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change -1.220842247
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.431505 0.000305467
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.849 1.069 0.765 0.733 0.914
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
-0.43 0.0405 -0.53 0.0106 -0.07 0.1699 DNA recombination; repair and maintenance (8hpi)
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
2LO1 NMR - A=1-70.
2M6Y NMR - A=1-67.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Rev inhibited by 24047968
capsid inhibited by 24047968
HIV-1 virus replication enhanced by expression of human gene 22404213

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04141 Protein processing in endoplasmic reticulum - Homo sapiens (human)