Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0000810
UniProt IDO43143
Primary gene name(s)DHX15
Synonym gene name(s)DBP1, DDX15
Protein namePre-mRNA-splicing factor ATP-dependent RNA helicase DHX15
Protein functionPre-mRNA processing factor involved in disassembly of spliceosomes after the release of mature mRNA. In cooperation with TFIP11 seem to be involved in the transition of the U2, U5 and U6 snRNP-containing IL complex to the snRNP-free IS complex leading to efficient debranching and turnover of excised introns. {ECO:0000269|PubMed:19103666}.
Subcellular locationNucleus. Nucleus, nucleolus.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: O43143
Gene Ontology
(Biological Process)
Complete annatation
mRNA processing [GO:0006397];
mRNA splicing, via spliceosome [GO:0000398];
response to alkaloid [GO:0043279];
response to toxic substance [GO:0009636];
RNA splicing [GO:0008380]
Gene Ontology
(Molecular Function)
Complete annatation
ATP binding [GO:0005524];
ATP-dependent RNA helicase activity [GO:0004004];
double-stranded RNA binding [GO:0003725];
poly(A RNA binding [GO:0044822];
RNA helicase activity [GO:0003724]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasm [GO:0005737];
nucleolus [GO:0005730];
nucleus [GO:0005634];
U12-type spliceosomal complex [GO:0005689]
Protein-protein interaction108029
Phylogenetic treeO43143
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
      unknown
Brass et al., Science, 2008
      unknown
Smith et al., J. Immunol, 2010
      unknown
Interferon-stimulated
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model


DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD31.217532714028530.0002452782203403720.00106261377895087
AZA vs. DISU0.08673179998061210.7312750435160850.974560663114398
AZA vs. IL70.3612175932695740.05992080168290440.703798825350824
AZA vs. SAHA-0.1595983744853480.5122286832261820.828428950446709
DISU vs. CD3-1.143512280021370.001805312175431670.00687203932637804
DISU vs. IL70.2655888460585670.2910456412932350.676904802665525
DISU vs. SAHA-0.245233840580430.3996987075839720.760071021924645
DMSO vs. AZA0.02663295800948930.873299604008611
DMSO vs. CD3-1.201993076787580.0002075785135564610.000859112721628596
DMSO vs. DISU-0.06182402806237370.7996185571427230.976466405242115
DMSO vs. IL70.341796466889890.05689775535346090.46565345382922
DMSO vs. SAHA-0.1932524188782870.41162334335890.75269603411201
HIV vs. Mock in Activation0.003605011973882520.9953769364216240.999983755607037
HIV vs. Mock in Latency0.09098869858518690.5802408736094240.999834320637052
IL7 vs. CD3-0.8482794987299920.00863399127745090.0263868918339451
SAHA vs. CD3-1.401802677382529.86551918775724e-050.000476975855500487
SAHA vs. IL7-0.5244125139070580.03144980237267150.134871784058506
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change -1.083037439
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.323499 0.0118205
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.025 0.931 0.836 0.792 0.804
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Envelope surface glycoprotein gp120 complexes with 23125841
Gag-Pol complexes with 23125841
Pr55(Gag) complexes with 23125841
HIV-1 virus replication enhanced by expression of human gene 18854154
Nef complexes with 23125841

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa03040 Spliceosome - Homo sapiens (human)
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