Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0000802
UniProt IDQ15392
Primary gene name(s)DHCR24
Synonym gene name(s)KIAA0018
Protein nameDelta(24-sterol reductase
Protein functionCatalyzes the reduction of the delta-24 double bond of sterol intermediates. Protects cells from oxidative stress by reducing caspase 3 activity during apoptosis induced by oxidative stress. Also protects against amyloid-beta peptide-induced apoptosis. {ECO:0000269|PubMed:11007892, ECO:0000269|PubMed:11519011, ECO:0000269|PubMed:22010141}.
Subcellular locationEndoplasmic reticulum membrane;
Single-pass membrane protein. Golgi apparatus membrane;
Single-pass membrane protein.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q15392
Gene Ontology
(Biological Process)
Complete annatation
amyloid precursor protein catabolic process [GO:0042987];
apoptotic process [GO:0006915];
cell cycle arrest [GO:0007050];
cholesterol biosynthetic process [GO:0006695];
cholesterol biosynthetic process via desmosterol [GO:0033489];
cholesterol biosynthetic process via lathosterol [GO:0033490];
male genitalia development [GO:0030539];
membrane organization [GO:0061024];
negative regulation of apoptotic process [GO:0043066];
negative regulation of cell proliferation [GO:0008285];
negative regulation of cysteine-type endopeptidase activity involved in apoptotic process [GO:0043154];
oxidation-reduction process [GO:0055114];
plasminogen activation [GO:0031639];
protein localization [GO:0008104];
Ras protein signal transduction [GO:0007265];
regulation of neuron death [GO:1901214];
response to hormone [GO:0009725];
response to oxidative stress [GO:0006979];
skin development [GO:0043588];
tissue development [GO:0009888]
Gene Ontology
(Molecular Function)
Complete annatation
delta24(24-1 sterol reductase activity [GO:0000246];
delta24-sterol reductase activity [GO:0050614];
enzyme binding [GO:0019899];
flavin adenine dinucleotide binding [GO:0050660];
oxidoreductase activity, acting on CH-OH group of donors [GO:0016614];
oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor [GO:0016628];
peptide antigen binding [GO:0042605]
Gene Ontology
(Cellular Component)
Complete annatation
cytoskeleton [GO:0005856];
cytosol [GO:0005829];
endoplasmic reticulum [GO:0005783];
endoplasmic reticulum membrane [GO:0005789];
Golgi membrane [GO:0000139];
integral component of membrane [GO:0016021];
membrane [GO:0016020];
nucleus [GO:0005634]
Protein-protein interaction108064
Phylogenetic treeQ15392
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.527448105783360.1078706253576570.187177764838891
AZA vs. DISU-0.4164827235835470.1682999579719750.74908949851251
AZA vs. IL70.2205215036219960.2967085410371380.999311006273513
AZA vs. SAHA-0.5018793624395580.0579027967460590.295372845397663
DISU vs. CD3-0.9542272146104830.009087734734014810.0267294660254711
DISU vs. IL70.6268400562988880.02599463930764420.205813648930009
DISU vs. SAHA-0.08292890315993350.7977416333094120.946755089663451
DMSO vs. AZA0.001010342711034040.9961554558784311
DMSO vs. CD3-0.5348198981687030.09497007844343440.164017451765487
DMSO vs. DISU0.4163906854010320.1417287710013690.633831026062851
DMSO vs. IL70.2266018459227490.2103831226485570.719866051541493
DMSO vs. SAHA-0.5083449586152840.03565927532349120.206224458327067
HIV vs. Mock in Activation0.2612710731327890.6755300111737050.999983755607037
HIV vs. Mock in Latency0.3467183599515450.06224494720754890.972826064385733
IL7 vs. CD3-0.3022126242056320.3468877498148640.484308809856203
SAHA vs. CD3-1.051507473411350.003351853976490960.0100964300271275
SAHA vs. IL7-0.7235444963966370.003389703384233940.0290816100250099
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.32527 0.00976514
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.008 1.142 1.639 1.967 1.048
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
200862_at 1.53 No upregulated in CD8+ cells

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Envelope surface glycoprotein gp160; precursor interacts with 22190034

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa00100 Steroid biosynthesis - Homo sapiens (human)
hsa01100 Metabolic pathways - Homo sapiens (human)