Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0000800
UniProt IDP52824
Primary gene name(s)DGKQ
Synonym gene name(s)DAGK4
Protein nameDiacylglycerol kinase theta
Protein functionPhosphorylates diacylglycerol, DAG to generate phosphatidic acid, PA. May regulate the activity of protein kinase C by controlling the balance between these two signaling lipids. Activated in the nucleus in response to alpha-thrombin and nerve growth factor, By similarity. May be involved in cAMP-induced activation of NR5A1 and subsequent steroidogenic gene transcription by delivering PA as ligand for NR5A1. Acts synergistically with NR5A1 on CYP17 transcriptional activity. {ECO:0000250, ECO:0000269|PubMed:17664281}.
Subcellular locationCytoplasm {ECO:0000269|PubMed:15632189}. Cell membrane {ECO:0000269|PubMed:10066731, ECO:0000269|PubMed:15632189}. Cytoplasm, cytoskeleton {ECO:0000269|PubMed:10066731}. Nucleus {ECO:0000269|PubMed:12799190}. Nucleus speckle {ECO:0000269|PubMed:12799190}. Note=Translocates to the nucleus in response to thrombin stimulation, PubMed:15632189. Translocates to the plasma membrane in response to steroid hormone receptor stimulation, PubMed:15632189. Translocation to the plasma membrane is dependent on G-protein coupled receptor stimulation and subsequent activation of PRKCE and probably PRKCH, PubMed:15632189.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P52824
Gene Ontology
(Biological Process)
Complete annatation
cAMP-mediated signaling [GO:0019933];
diacylglycerol metabolic process [GO:0046339];
glycerolipid metabolic process [GO:0046486];
G-protein coupled receptor signaling pathway [GO:0007186];
lipid phosphorylation [GO:0046834];
platelet activation [GO:0030168];
protein kinase C-activating G-protein coupled receptor signaling pathway [GO:0007205];
protein kinase C signaling [GO:0070528];
regulation of transcription from RNA polymerase II promoter [GO:0006357];
response to ATP [GO:0033198];
thrombin-activated receptor signaling pathway [GO:0070493]
Gene Ontology
(Molecular Function)
Complete annatation
activating transcription factor binding [GO:0033613];
ATP binding [GO:0005524];
diacylglycerol kinase activity [GO:0004143];
kinase binding [GO:0019900];
metal ion binding [GO:0046872];
phospholipase binding [GO:0043274]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasm [GO:0005737];
cytoskeleton [GO:0005856];
cytosol [GO:0005829];
nuclear speck [GO:0016607];
nucleus [GO:0005634];
plasma membrane [GO:0005886]
Protein-protein interaction107979
Phylogenetic treeP52824
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.2176534295792170.5108418072863280.626783435315821
AZA vs. DISU-0.2426445457971120.3389001262841350.880262543573058
AZA vs. IL7-0.2200462072451950.2551853547760520.999311006273513
AZA vs. SAHA0.0901694371712440.7124027477360410.918972149307812
DISU vs. CD3-0.03749450295179940.9187596266848780.947225402684348
DISU vs. IL70.01310078674456090.9586196614982070.993209030727524
DISU vs. SAHA0.3354322256493240.2513081597356770.629565371571873
DMSO vs. AZA0.09023450315983440.5924977203699761
DMSO vs. CD30.2927647130987150.3662595867464650.480510378327571
DMSO vs. DISU0.3302234216817990.177239412609110.690311335740057
DMSO vs. IL7-0.3022929892592890.09468507527394740.564669579669709
DMSO vs. SAHA-0.003910953717135170.9868023959168760.9961149026161
HIV vs. Mock in Activation0.2094256384257130.7589091492452380.999983755607037
HIV vs. Mock in Latency-0.02548118897075520.8781037524993540.999834320637052
IL7 vs. CD30.002667170563428680.9937363800890840.996055666614787
SAHA vs. CD30.2849650638038080.4231712862050430.537841889441679
SAHA vs. IL70.3073992980938540.20900376720840.443923176962015
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.029594 0.886681
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa00561 Glycerolipid metabolism - Homo sapiens (human)
hsa00564 Glycerophospholipid metabolism - Homo sapiens (human)
hsa01100 Metabolic pathways - Homo sapiens (human)
hsa04070 Phosphatidylinositol signaling system - Homo sapiens (human)
hsa04072 Phospholipase D signaling pathway - Homo sapiens (human)
hsa05231 Choline metabolism in cancer - Homo sapiens (human)