Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0000799
UniProt IDQ16760
Primary gene name(s)DGKD
Synonym gene name(s)KIAA0145
Protein nameDiacylglycerol kinase delta
Protein functionMay function as signaling molecule. {ECO:0000269|PubMed:17880279}.; FUNCTION: Isoform 2 may be involved in cell growth and tumorigenesis. Involved in clathrin-dependent endocytosis. {ECO:0000269|PubMed:17880279}.
Subcellular locationIsoform 2: Cytoplasm {ECO:0000269|PubMed:12200442}.;
SUBCELLULAR LOCATION: Isoform 1: Membrane {ECO:0000269|PubMed:12200442};
Peripheral membrane protein {ECO:0000269|PubMed:12200442}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q16760
Gene Ontology
(Biological Process)
Complete annatation
cell growth [GO:0016049];
diacylglycerol metabolic process [GO:0046339];
endocytosis [GO:0006897];
epidermal growth factor receptor signaling pathway [GO:0007173];
lipid phosphorylation [GO:0046834];
multicellular organism development [GO:0007275];
platelet activation [GO:0030168];
protein homooligomerization [GO:0051260];
protein kinase C-activating G-protein coupled receptor signaling pathway [GO:0007205];
protein transport [GO:0015031];
response to organic substance [GO:0010033];
second-messenger-mediated signaling [GO:0019932];
signal transduction [GO:0007165]
Gene Ontology
(Molecular Function)
Complete annatation
ATP binding [GO:0005524];
diacylglycerol binding [GO:0019992];
diacylglycerol kinase activity [GO:0004143];
metal ion binding [GO:0046872];
protein heterodimerization activity [GO:0046982];
protein homodimerization activity [GO:0042803]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasm [GO:0005737];
cytoplasmic, membrane-bounded vesicle [GO:0016023];
plasma membrane [GO:0005886]
Protein-protein interaction114097
Phylogenetic treeQ16760
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      Yes - >4 <5 SD (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-1.113034560130690.001597410531558710.00546316576620214
AZA vs. DISU-0.005842027230252440.9815856056931720.998432150441561
AZA vs. IL7-0.2352798131197880.2218517557110720.989265982093417
AZA vs. SAHA-0.07476932102765990.7592378552530310.935410823872839
DISU vs. CD31.093515951841430.003213314269154720.0112113504091623
DISU vs. IL7-0.2380990608567370.3448887741004360.725928220383342
DISU vs. SAHA-0.06725998302474290.8175066200252050.953167435432452
DMSO vs. AZA0.1156669542580970.490357846148361
DMSO vs. CD31.211353764568460.0007989983766282240.00281236227661313
DMSO vs. DISU0.1180956233650780.6285746577635520.945537968267771
DMSO vs. IL7-0.3427962820665530.05713210312522030.466367296819318
DMSO vs. SAHA-0.1953424387519520.407396768312310.74924797009086
HIV vs. Mock in Activation0.1979595267841310.8002908127602080.999983755607037
HIV vs. Mock in Latency-0.2757577083715680.09520988967781930.999834320637052
IL7 vs. CD30.8853450504015710.01271190194198930.0362330351691482
SAHA vs. CD31.013492392376340.005319688041062530.0150719493849995
SAHA vs. IL70.1563540758789160.5210138486283250.743186086222934
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.334087 0.0107117
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
208072_s_at 1.46 No downregulated in CD8+ cells

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

Drugbank ID Drug Name Drug Status Pharmacological Action Drug Action
DB00144 Phosphatidyl serine approved, nutraceutical unknown unknown

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
1R79 NMR - A=216-286.
3BQ7 X-ray 2.9Å A/B/C/D/E/F=1141-1208.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
HIV-1 virus replication inhibited by expression of human gene 22082156

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa00561 Glycerolipid metabolism - Homo sapiens (human)
hsa00564 Glycerophospholipid metabolism - Homo sapiens (human)
hsa01100 Metabolic pathways - Homo sapiens (human)
hsa04070 Phosphatidylinositol signaling system - Homo sapiens (human)
hsa04072 Phospholipase D signaling pathway - Homo sapiens (human)
hsa05231 Choline metabolism in cancer - Homo sapiens (human)