Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0000795
UniProt IDO75140
Primary gene name(s)DEPDC5
Synonym gene name(s)KIAA0645
Protein nameDEP domain-containing protein 5
Protein functionAs a component of the GATOR1 complex functions as an inhibitor of the amino acid-sensing branch of the TORC1 pathway. The GATOR1 complex strongly increases GTP hydrolysis by RRAGA and RRAGB within RRAGC-containing heterodimers, thereby deactivating RRAGs, releasing mTORC1 from lysosomal surface and inhibiting mTORC1 signaling. The GATOR1 complex is negatively regulated by GATOR2 the other GATOR subcomplex in this amino acid-sensing branch of the TORC1 pathway. {ECO:0000269|PubMed:23723238, ECO:0000269|PubMed:25457612}.
Subcellular locationCytoplasm, cytosol {ECO:0000250}. Cytoplasm, perinuclear region {ECO:0000250}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: O75140
Gene Ontology
(Biological Process)
Complete annatation
cellular response to amino acid starvation [GO:0034198];
intracellular signal transduction [GO:0035556];
negative regulation of TORC1 signaling [GO:1904262];
negative regulation of TOR signaling [GO:0032007];
regulation of autophagy [GO:0010506]
Gene Ontology
(Molecular Function)
Complete annatation
GTPase activator activity [GO:0005096];
protein complex binding [GO:0032403]
Gene Ontology
(Cellular Component)
Complete annatation
cytosol [GO:0005829];
Iml1 complex [GO:1990130];
lysosomal membrane [GO:0005765];
lysosome [GO:0005764];
perinuclear region of cytoplasm [GO:0048471]
Protein-protein interaction115034
Phylogenetic treeO75140
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.1901179571969470.5646771095876590.673590296454705
AZA vs. DISU0.2205174953366810.3896308721378580.901458726582074
AZA vs. IL7-0.01648120660517130.9335205717167380.999311006273513
AZA vs. SAHA-0.1829839090411950.4622522001223890.800826303257659
DISU vs. CD30.01803094566588170.9605034936063620.974451908075195
DISU vs. IL7-0.246625337229320.3336898899585910.71426374660371
DISU vs. SAHA-0.4017785248635490.173286047907260.536722207354818
DMSO vs. AZA0.09633772668676630.5790913072826181
DMSO vs. CD3-0.1048084231297450.7446115703667230.815526958020696
DMSO vs. DISU-0.1261678026752030.6095503352741580.939289650701039
DMSO vs. IL7-0.1050765738974050.5695085373921880.903436831021822
DMSO vs. SAHA-0.2853445451954380.2344378913947670.581657942676482
HIV vs. Mock in Activation0.206496158617920.7414709318198890.999983755607037
HIV vs. Mock in Latency0.165039640510610.3337245148273070.999834320637052
IL7 vs. CD3-0.200516608925540.5353671438586180.660954128870584
SAHA vs. CD3-0.3970438712574920.2646549353875330.376046565744873
SAHA vs. IL7-0.1700371562521110.49261374141980.722820281327394
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.229966 0.232113
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
HIV-1 virus replication enhanced by expression of human gene 18187620

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04150 mTOR signaling pathway - Homo sapiens (human)