Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0000769
UniProt IDQ96HY6
Primary gene name(s)DDRGK1
Synonym gene name(s)C20orf116, UFBP1
Protein nameDDRGK domain-containing protein 1
Protein functionProtein which interacts with the E3 UFM1-protein ligase UFL1 and one of its substrates TRIP4 and is required for TRIP4 ufmylation. Through TRIP4 ufmylation may regulate nuclear receptors-mediated transcription, PubMed:25219498. May play a role in NF-kappa-B-mediated transcription through regulation of the phosphorylation and the degradation of NFKBIA, the inhibitor of NF-kappa-B, PubMed:23675531. May also play a role in the cellular response to endoplasmic reticulum stress, By similarity. {ECO:0000250|UniProtKB:Q80WW9, ECO:0000269|PubMed:23675531, ECO:0000269|PubMed:25219498}.
Subcellular locationEndoplasmic reticulum {ECO:0000269|PubMed:20018847}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q96HY6
Gene Ontology
(Biological Process)
Complete annatation
negative regulation of apoptotic process [GO:0043066];
negative regulation of gene expression [GO:0010629];
positive regulation of cell proliferation [GO:0008284];
positive regulation of gene expression [GO:0010628];
positive regulation of I-kappaB phosphorylation [GO:1903721];
positive regulation of NF-kappaB transcription factor activity [GO:0051092];
positive regulation of proteasomal protein catabolic process [GO:1901800];
positive regulation of proteasomal ubiquitin-dependent protein catabolic process [GO:0032436];
positive regulation of protein localization to endoplasmic reticulum [GO:1905552];
positive regulation of RNA polymerase II regulatory region sequence-specific DNA binding [GO:1905636];
protein K69-linked ufmylation [GO:1990592];
regulation of intracellular estrogen receptor signaling pathway [GO:0033146];
response to endoplasmic reticulum stress [GO:0034976]
Gene Ontology
(Molecular Function)
Complete annatation
ubiquitin-like protein ligase binding [GO:0044389]
Gene Ontology
(Cellular Component)
Complete annatation
endoplasmic reticulum [GO:0005783];
endoplasmic reticulum membrane [GO:0005789]
Protein-protein interaction122441
Phylogenetic treeQ96HY6
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.1502714582907720.647982731911730.7438796355681
AZA vs. DISU0.2227104349995430.3807308374318370.899651703517147
AZA vs. IL7-0.01446009491281670.9405992156278720.999311006273513
AZA vs. SAHA-0.7285089143608930.00336982893057780.0476517835830486
DISU vs. CD30.3609932421191670.322380433984770.455271000738844
DISU vs. IL7-0.2474256297755250.3281875870522350.708652576453954
DISU vs. SAHA-0.9487174762323520.001419986406522520.026355437355543
DMSO vs. AZA-0.08238464568275560.7002695608587831
DMSO vs. CD30.06247249295781880.8458302238544250.893766733700421
DMSO vs. DISU-0.3054271488587510.2401907671512440.757157858551333
DMSO vs. IL70.07435720447122320.733918589184420.946333083680773
DMSO vs. SAHA-0.6534542925759570.013963122982990.111935226849278
HIV vs. Mock in Activation0.3528881871570040.5747464092685520.999983755607037
HIV vs. Mock in Latency0.1038537832412340.5341102285452510.999834320637052
IL7 vs. CD30.1406603496274420.6627058996541760.768263807528642
SAHA vs. CD3-0.6014744028666590.09072818140955670.161121104624325
SAHA vs. IL7-0.7162154752936480.003802022365204880.0316374352412855
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.253259 0.100352
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.972 1.061 1.11 1.196 1.087
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
not found