Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0000766
UniProt IDO94830
Primary gene name(s)DDHD2
Synonym gene name(s)KIAA0725, SAMWD1
Protein namePhospholipase DDHD2
Protein functionPhospholipase that hydrolyzes preferentially phosphatidic acid, including 1,2-dioleoyl-sn-phosphatidic acid, and phosphatidylethanolamine. Specifically binds to phosphatidylinositol 3-phosphate, PI(3P, phosphatidylinositol 4-phosphate, PI(4P, phosphatidylinositol 5-phosphate, PI(5P and possibly phosphatidylinositol 4,5-bisphosphate, PI(4,5P2. May be involved in the maintenance of the endoplasmic reticulum and/or Golgi structures. May regulate the transport between Golgi apparatus and plasma membrane. {ECO:0000269|PubMed:11788596, ECO:0000269|PubMed:20932832, ECO:0000269|PubMed:22922100}.
Subcellular locationCytoplasm, cytosol. Endoplasmic reticulum-Golgi intermediate compartment. Golgi apparatus, cis-Golgi network. Note=Cycles between the Golgi apparatus and the cytosol. DDHD2 recruitment to the Golgi/endoplasmic reticulum-Golgi intermediate compartment, ERGIC is regulated by the levels of phosphoinositides, including PI(4P.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: O94830
Gene Ontology
(Biological Process)
Complete annatation
ER to Golgi vesicle-mediated transport [GO:0006888];
lipid particle organization [GO:0034389];
locomotory behavior [GO:0007626];
phosphatidic acid biosynthetic process [GO:0006654];
positive regulation of mitochondrial fission [GO:0090141];
triglyceride catabolic process [GO:0019433];
visual learning [GO:0008542]
Gene Ontology
(Molecular Function)
Complete annatation
hydrolase activity [GO:0016787];
metal ion binding [GO:0046872]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasm [GO:0005737];
cytosol [GO:0005829];
endoplasmic reticulum-Golgi intermediate compartment [GO:0005793];
Golgi apparatus [GO:0005794];
microtubule organizing center [GO:0005815]
Protein-protein interaction116862
Phylogenetic treeO94830
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.5544044344113970.09136784706274540.163511777597922
AZA vs. DISU0.1949022737455280.4422987105231510.91814655876178
AZA vs. IL70.06225063634746670.7460310943231450.999311006273513
AZA vs. SAHA-0.4077079795664420.09541161126239780.389871528644808
DISU vs. CD30.7369834666363210.04442857260332920.0980550956694853
DISU vs. IL7-0.1417042210469310.5748514071856890.86399650681199
DISU vs. SAHA-0.6016405785393570.03965198460944850.244315233053556
DMSO vs. AZA-0.001522897175053520.9927475463309231
DMSO vs. CD30.541990457146160.09092426135615010.158063004538472
DMSO vs. DISU-0.1979632773078990.4186870013546330.876353539651597
DMSO vs. IL70.07084343704025840.6935125484506490.936954851917814
DMSO vs. SAHA-0.41307680137480.08046948345766690.330721457646713
HIV vs. Mock in Activation0.1487061148390790.8114346345187040.999983755607037
HIV vs. Mock in Latency0.1029650794282520.5327475121912070.999834320637052
IL7 vs. CD30.6242418395368090.0528714465570010.115013900123203
SAHA vs. CD30.1220873177343390.7302826799541460.804836017219574
SAHA vs. IL7-0.4735638397001110.05242762473531370.190524111682767
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.0422248 0.836663
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.001 1.043 1.034 1.068 1.049
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
212690_at 1.4 No downregulated in CD8+ cells

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
not found