Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0000761
UniProt IDQ8IWE4
Primary gene name(s)DCUN1D3
Synonym gene name(s)SCCRO3
Protein nameDCN1-like protein 3
Protein functionAntagonizes DCUN1D1-mediated CUL1 neddylation by sequestering CUL1 at the cell membrane, PubMed:25349211. When overexpressed in transformed cells, may promote mesenchymal to epithelial-like changes and inhibit colony formation in soft agar, PubMed:25349211. {ECO:0000269|PubMed:25349211}.
Subcellular locationCell membrane {ECO:0000269|PubMed:25349211}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q8IWE4
Gene Ontology
(Biological Process)
Complete annatation
negative regulation of cell growth [GO:0030308];
positive regulation of apoptotic process [GO:0043065];
positive regulation of ubiquitin-protein transferase activity [GO:0051443];
protein neddylation [GO:0045116];
response to gamma radiation [GO:0010332];
response to UV-C [GO:0010225]
Gene Ontology
(Molecular Function)
Complete annatation
cullin family protein binding [GO:0097602];
ubiquitin conjugating enzyme binding [GO:0031624];
ubiquitin-like protein binding [GO:0032182]
Gene Ontology
(Cellular Component)
Complete annatation
perinuclear region of cytoplasm [GO:0048471];
plasma membrane [GO:0005886];
ubiquitin ligase complex [GO:0000151]
Protein-protein interaction125841
Phylogenetic treeQ8IWE4
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.440509094260450.1866364792930270.289074608560734
AZA vs. DISU0.1088502337859120.6779449073720290.964884761087644
AZA vs. IL7-0.01254262124172580.9512755033742530.999311006273513
AZA vs. SAHA0.3132925981245940.2174636045640690.585967791750363
DISU vs. CD3-0.3444999311668440.3487659691511580.482453722949854
DISU vs. IL7-0.1301476629349060.6171463295150230.88391841050305
DISU vs. SAHA0.20631511867390.4917086548982330.819659455588017
DMSO vs. AZA-0.1509481154757060.4111276403579411
DMSO vs. CD3-0.6013029608690430.06546506151806490.120786450039852
DMSO vs. DISU-0.261295795477560.3016743529063580.808227572050797
DMSO vs. IL70.1459319621010230.4498711108014460.869350980045097
DMSO vs. SAHA0.4578383020434630.06314505391128530.289434035126819
HIV vs. Mock in Activation0.7084987517751070.2617741051814640.999983755607037
HIV vs. Mock in Latency0.08898063873629720.6182709166862270.999834320637052
IL7 vs. CD3-0.4467628386488480.172214596622140.288229026747225
SAHA vs. CD3-0.1519872624057190.6715129564497110.756443433524978
SAHA vs. IL70.3235572927773860.2015089541182420.434183243907716
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.503862 0.0279653
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
4GBA X-ray 2.4Å A/B=86-304.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
not found