Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0000758
UniProt IDQ13561
Primary gene name(s)DCTN2
Synonym gene name(s)DCTN50
Protein nameDynactin subunit 2
Protein functionModulates cytoplasmic dynein binding to an organelle, and plays a role in prometaphase chromosome alignment and spindle organization during mitosis. Involved in anchoring microtubules to centrosomes. May play a role in synapse formation during brain development.
Subcellular locationCytoplasm, cytoskeleton, microtubule organizing center, centrosome {ECO:0000269|PubMed:8647893}. Membrane {ECO:0000269|PubMed:8647893};
Peripheral membrane protein {ECO:0000269|PubMed:8647893}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q13561
Gene Ontology
(Biological Process)
Complete annatation
antigen processing and presentation of exogenous peptide antigen via MHC class II [GO:0019886];
cell proliferation [GO:0008283];
ER to Golgi vesicle-mediated transport [GO:0006888];
G2/M transition of mitotic cell cycle [GO:0000086];
melanosome transport [GO:0032402];
microtubule-based process [GO:0007017];
mitotic nuclear division [GO:0007067];
mitotic spindle organization [GO:0007052];
protein localization to centrosome [GO:0071539]
Gene Ontology
(Molecular Function)
Complete annatation
motor activity [GO:0003774];
spectrin binding [GO:0030507]
Gene Ontology
(Cellular Component)
Complete annatation
centrosome [GO:0005813];
cytoplasm [GO:0005737];
cytosol [GO:0005829];
dynactin complex [GO:0005869];
dynein complex [GO:0030286];
extracellular exosome [GO:0070062];
growth cone [GO:0030426];
kinetochore [GO:0000776];
membrane [GO:0016020];
microtubule [GO:0005874];
vesicle [GO:0031982]
Protein-protein interaction115794
Phylogenetic treeQ13561
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.09622552079786810.7724056748165650.842734336917289
AZA vs. DISU-0.03983928520181080.8748403476586580.991631204917382
AZA vs. IL7-0.06959270340200150.7177801783136740.999311006273513
AZA vs. SAHA0.08399689258715470.7306533719098760.925379678051543
DISU vs. CD30.04419545648320640.9043896463576920.937540026765412
DISU vs. IL7-0.0393196058493430.8759983051152240.976126113490129
DISU vs. SAHA0.1258161903594080.665802810517760.898517921545065
DMSO vs. AZA-0.03237424338160620.8470412003814821
DMSO vs. CD30.05171749655989110.8733839769615720.912639912112511
DMSO vs. DISU0.00538824668035910.9823789281131770.99735766500781
DMSO vs. IL7-0.0299234911731720.8680236428314560.97087523487789
DMSO vs. SAHA0.1105958348799960.639021500571650.884121307158001
HIV vs. Mock in Activation0.02420587166151030.9707575974295130.999983755607037
HIV vs. Mock in Latency-0.1045669596397610.5275014813905890.999834320637052
IL7 vs. CD30.03328481117168070.9183271377192480.948413529976402
SAHA vs. CD30.1560805567438280.6629708359936760.749119351781814
SAHA vs. IL70.1507581240790120.5360108879753770.754520385171463
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.299947 0.0801766
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.03 1.025 1.194 1.237 1.162
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
capsid upregulated by 25375884
Pr55(Gag) modulated by 19286658

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04962 Vasopressin-regulated water reabsorption - Homo sapiens (human)
hsa05016 Huntington's disease - Homo sapiens (human)