Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0000750
UniProt IDQ9UBU7
Primary gene name(s)DBF4
Synonym gene name(s)ASK, DBF4A, ZDBF1
Protein nameProtein DBF4 homolog A
Protein functionRegulatory subunit for CDC7 which activates its kinase activity thereby playing a central role in DNA replication and cell proliferation. Required for progression of S phase. The complex CDC7-DBF4A selectively phosphorylates MCM2 subunit at 'Ser-40' and 'Ser-53' and then is involved in regulating the initiation of DNA replication during cell cycle. {ECO:0000269|PubMed:10373557, ECO:0000269|PubMed:10523313, ECO:0000269|PubMed:17062569}.
Subcellular locationNucleus {ECO:0000269|PubMed:10373557}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q9UBU7
Gene Ontology
(Biological Process)
Complete annatation
DNA replication [GO:0006260];
G1/S transition of mitotic cell cycle [GO:0000082]
Gene Ontology
(Molecular Function)
Complete annatation
enzyme activator activity [GO:0008047];
nucleic acid binding [GO:0003676];
zinc ion binding [GO:0008270]
Gene Ontology
(Cellular Component)
Complete annatation
nucleoplasm [GO:0005654]
Protein-protein interaction116129
Phylogenetic treeQ9UBU7
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.9543882263081740.004167247981577190.0124694781771553
AZA vs. DISU0.3466348400618810.1748591055838680.76025598363942
AZA vs. IL70.03914937332576170.8556450570766060.999311006273513
AZA vs. SAHA-0.3654483212625180.1378084554032870.471155512984612
DISU vs. CD3-0.6205126876435550.08817830186043740.168851590732245
DISU vs. IL7-0.3154766104757190.2140720987701850.594525851424971
DISU vs. SAHA-0.7118751696726340.01586448912579220.139739045320278
DMSO vs. AZA-0.1895129813599640.2662627917006671
DMSO vs. CD3-1.156173649473530.0004054871872536390.00155160388010568
DMSO vs. DISU-0.5380899346364530.02948948929579340.346503199876135
DMSO vs. IL70.2357211828692860.1959049114132050.705847643296861
DMSO vs. SAHA-0.1837295813666980.4398677787472780.773360626866569
HIV vs. Mock in Activation-0.3514212286059630.5733611722239870.999983755607037
HIV vs. Mock in Latency0.01668385555886850.9208686615233920.999834320637052
IL7 vs. CD3-0.9048431820597660.005465456190271060.0180580493546593
SAHA vs. CD3-1.345791059406180.0002039584374305960.000897368164315629
SAHA vs. IL7-0.409456324579440.09571011832148730.280084719152395
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
1.8 0.008375107 1.2 0.078846513 1.2 0.272929175
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.783716 0.000305467
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
4F99 X-ray 2.3Å B=210-350.
4F9A X-ray 2.1Å B/D=210-350.
4F9B X-ray 2.5Å B/D=210-350.
4F9C X-ray 2.0Å B=210-350.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04110 Cell cycle - Homo sapiens (human)