Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0000741
UniProt IDO43739
Primary gene name(s)CYTH3
Synonym gene name(s)ARNO3, GRP1, PSCD3
Protein nameCytohesin-3
Protein functionPromotes guanine-nucleotide exchange on ARF1 and ARF6. Promotes the activation of ARF factors through replacement of GDP with GTP. {ECO:0000269|PubMed:23940353, ECO:0000269|PubMed:9707577}.
Subcellular locationCytoplasm, cytosol. Cell membrane;
Peripheral membrane protein. Note=Translocates from the cytosol to membranes enriched in phosphatidylinositol 3,4,5-trisphosphate.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: O43739
Gene Ontology
(Biological Process)
Complete annatation
establishment of epithelial cell polarity [GO:0090162];
Golgi vesicle transport [GO:0048193];
positive regulation of cell adhesion [GO:0045785];
regulation of ARF protein signal transduction [GO:0032012]
Gene Ontology
(Molecular Function)
Complete annatation
ARF guanyl-nucleotide exchange factor activity [GO:0005086];
phosphatidylinositol-3,4,5-trisphosphate binding [GO:0005547]
Gene Ontology
(Cellular Component)
Complete annatation
cytosol [GO:0005829];
extrinsic component of cytoplasmic side of plasma membrane [GO:0031234];
Golgi membrane [GO:0000139];
plasma membrane [GO:0005886];
ruffle [GO:0001726]
Protein-protein interaction114686
Phylogenetic treeO43739
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.279050244092460.6259120567841550.726567045583086
AZA vs. DISU0.4203024568073410.1428880842798550.718797155225174
AZA vs. IL7-0.02361075064195210.9358910321607740.999311006273513
AZA vs. SAHA1.008712691090469.48259800690465e-050.00364644597847145
DISU vs. CD30.6842612013457620.2501907239634740.376986702621492
DISU vs. IL7-0.4506406035420290.1602748610736020.518819740323073
DISU vs. SAHA0.5907208756701920.0496430533719260.278789029735687
DMSO vs. AZA0.2785842072218270.4246226637979521
DMSO vs. CD30.5346119849349810.385817724602510.500330896198399
DMSO vs. DISU-0.1469438154107430.6863519242678960.957954410639349
DMSO vs. IL7-0.2917626308589950.434319581418530.860024653866327
DMSO vs. SAHA0.7270317078375630.02030294556620150.143050814692951
HIV vs. Mock in Activation-0.1810051759116540.8748623057844880.999983755607037
HIV vs. Mock in Latency-1.162459805348240.03567956421715270.723867767172758
IL7 vs. CD30.2654309060227950.6506340213598570.758762599919313
SAHA vs. CD31.263553625892680.0198884464483640.0458776806944505
SAHA vs. IL71.030049884833786.04834469249971e-050.00130433115729243
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.140909 0.521704
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

Drugbank ID Drug Name Drug Status Pharmacological Action Drug Action
DB03344 Inositol-(1,3,4,5,6)-Pentakisphosphate experimental unknown unknown
DB01863 Inositol 1,3,4,5-Tetrakisphosphate experimental unknown unknown

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
4KAX X-ray 1.8Å B=247-400.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04072 Phospholipase D signaling pathway - Homo sapiens (human)
hsa04144 Endocytosis - Homo sapiens (human)