Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0000737
UniProt IDO15528
Primary gene name(s)CYP27B1
Synonym gene name(s)CYP1ALPHA, CYP27B
Protein name25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial
Protein functionCatalyzes the conversion of 25-hydroxyvitamin D3, 25(OHD3 to 1-alpha,25-dihydroxyvitamin D3, 1alpha,25(OH(2D3, and of 24,25-dihydroxyvitamin D3, 24,25(OH(2D3 to 1-alpha,24,25-trihydroxyvitamin D3, 1alpha,24,25(OH(3D3. Is also active with 25-hydroxy-24-oxo-vitamin D3. Plays an important role in normal bone growth, calcium metabolism, and tissue differentiation. {ECO:0000269|PubMed:10518789}.
Subcellular locationMitochondrion membrane.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: O15528
Gene Ontology
(Biological Process)
Complete annatation
bone mineralization [GO:0030282];
calcitriol biosynthetic process from calciol [GO:0036378];
calcium ion homeostasis [GO:0055074];
calcium ion transport [GO:0006816];
decidualization [GO:0046697];
G1 to G0 transition [GO:0070314];
negative regulation of calcidiol 1-monooxygenase activity [GO:0010956];
negative regulation of cell growth [GO:0030308];
negative regulation of cell proliferation [GO:0008285];
positive regulation of keratinocyte differentiation [GO:0045618];
positive regulation of vitamin D 24-hydroxylase activity [GO:0010980];
positive regulation of vitamin D receptor signaling pathway [GO:0070564];
regulation of bone mineralization [GO:0030500];
response to estrogen [GO:0043627];
response to interferon-gamma [GO:0034341];
response to lipopolysaccharide [GO:0032496];
response to vitamin D [GO:0033280];
vitamin D catabolic process [GO:0042369];
vitamin D metabolic process [GO:0042359];
vitamin metabolic process [GO:0006766]
Gene Ontology
(Molecular Function)
Complete annatation
calcidiol 1-monooxygenase activity [GO:0004498];
heme binding [GO:0020037];
iron ion binding [GO:0005506]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasm [GO:0005737];
mitochondrial outer membrane [GO:0005741];
mitochondrion [GO:0005739]
Protein-protein interactionunknown
Phylogenetic treeO15528
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD32.873453774135823.85247389544929e-141.14751949707928e-12
AZA vs. DISUunknownunknownunknown
AZA vs. IL7unknownunknownunknown
AZA vs. SAHAunknownunknownunknown
DISU vs. CD3-2.635791789734337.37192529243202e-111.86450338688121e-09
DISU vs. IL7unknownunknownunknown
DISU vs. SAHAunknownunknownunknown
DMSO vs. AZAunknownunknownunknown
DMSO vs. CD3-2.843518080295551.06581410364015e-143.11776494589104e-13
DMSO vs. DISUunknownunknownunknown
DMSO vs. IL7unknownunknownunknown
DMSO vs. SAHAunknownunknownunknown
HIV vs. Mock in Activation0.2181655269253850.7333551365668220.999983755607037
HIV vs. Mock in Latencyunknownunknownunknown
IL7 vs. CD3-2.503055844958264.52426984764998e-121.19639156866014e-10
SAHA vs. CD3-3.334314411362188.88178419700125e-164.53723687623081e-14
SAHA vs. IL7unknownunknownunknown
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.555897 0.00710819
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

Drugbank ID Drug Name Drug Status Pharmacological Action Drug Action
DB00146 Calcidiol approved, nutraceutical unknown substrate
DB00153 Ergocalciferol approved, nutraceutical unknown substrate
DB01436 Alfacalcidol approved, nutraceutical unknown unknown
DB01285 Corticotropin approved, vet_approved unknown inducer

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
matrix activates 9282826

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa00100 Steroid biosynthesis - Homo sapiens (human)
hsa01100 Metabolic pathways - Homo sapiens (human)
hsa05152 Tuberculosis - Homo sapiens (human)