Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0000733
UniProt IDQ9UHQ9
Primary gene name(s)CYB5R1
Synonym gene name(s)NQO3A2
Protein nameNADH-cytochrome b5 reductase 1
Protein functionNADH-cytochrome b5 reductases are involved in desaturation and elongation of fatty acids, cholesterol biosynthesis, drug metabolism, and, in erythrocyte, methemoglobin reduction. {ECO:0000250}.
Subcellular locationMembrane {ECO:0000305};
Single-pass membrane protein {ECO:0000305}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q9UHQ9
Gene Ontology
(Biological Process)
Complete annatation
bicarbonate transport [GO:0015701];
platelet degranulation [GO:0002576];
positive regulation of protein targeting to mitochondrion [GO:1903955];
sterol biosynthetic process [GO:0016126]
Gene Ontology
(Molecular Function)
Complete annatation
cytochrome-b5 reductase activity, acting on NAD(PH [GO:0004128]
Gene Ontology
(Cellular Component)
Complete annatation
endoplasmic reticulum membrane [GO:0005789];
extracellular exosome [GO:0070062];
integral component of membrane [GO:0016021];
membrane [GO:0016020];
mitochondrion [GO:0005739];
plasma membrane [GO:0005886];
platelet alpha granule membrane [GO:0031092]
Protein-protein interaction119690
Phylogenetic treeQ9UHQ9
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.6022577706971260.07006016666679740.131320337271729
AZA vs. DISU-0.02850931224487730.9115119398990930.994271142470137
AZA vs. IL7-0.08322353141251670.6690788106486190.999311006273513
AZA vs. SAHA0.3609450320618290.1443793782737150.4840396698868
DISU vs. CD30.5602490143242490.1362173116340390.23784776503782
DISU vs. IL7-0.06460862129192490.7998648745092060.955495589373324
DISU vs. SAHA0.3922888278536660.1816072019687850.547181555552091
DMSO vs. AZA0.0329884173004260.8464058962741051
DMSO vs. CD30.6232647896592830.05486098408971680.104208892360558
DMSO vs. DISU0.05930680982024370.8105389542294570.978779334458453
DMSO vs. IL7-0.1087893659846150.5503625668041450.897857969069977
DMSO vs. SAHA0.3228313605657310.1764355540668330.507841908965096
HIV vs. Mock in Activation-0.04334602399240170.9446542843733520.999983755607037
HIV vs. Mock in Latency0.01498030868357730.9287125445042790.999834320637052
IL7 vs. CD30.5251410110430080.1077967674865380.200917605579892
SAHA vs. CD30.9397562470306630.01047519620624390.0266760770099587
SAHA vs. IL70.4420089624305080.07295714256985030.23610498977569
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.0960656 0.584186
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.082 1.236 1.706 2.112 1.396
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
202263_at 1.47 No downregulated in CD8+ cells

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

Drugbank ID Drug Name Drug Status Pharmacological Action Drug Action
DB03147 Flavin adenine dinucleotide approved unknown unknown

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa00520 Amino sugar and nucleotide sugar metabolism - Homo sapiens (human)