Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0000718
UniProt IDQ9UBX1
Primary gene name(s)CTSF
Synonym gene name(s)unknown
Protein nameCathepsin F
Protein functionThiol protease which is believed to participate in intracellular degradation and turnover of proteins. Has also been implicated in tumor invasion and metastasis.
Subcellular locationLysosome.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q9UBX1
Gene Ontology
(Biological Process)
Complete annatation
antigen processing and presentation of exogenous peptide antigen via MHC class II [GO:0019886];
proteolysis [GO:0006508];
proteolysis involved in cellular protein catabolic process [GO:0051603]
Gene Ontology
(Molecular Function)
Complete annatation
cysteine-type endopeptidase activity [GO:0004197]
Gene Ontology
(Cellular Component)
Complete annatation
extracellular exosome [GO:0070062];
extracellular space [GO:0005615];
extracellular vesicle [GO:1903561];
lysosomal lumen [GO:0043202];
lysosome [GO:0005764]
Protein-protein interaction114261
Phylogenetic treeQ9UBX1
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-2.090565473140331.96664543306024e-061.42987361153324e-05
AZA vs. DISU0.3710845349294270.1541534639701640.736295207806145
AZA vs. IL7-0.9007496651359690.001306916437160410.0980057286433774
AZA vs. SAHA0.6169754752222490.2168860051360110.585819449975489
DISU vs. CD32.448978374279211.99050526061839e-082.8795466697276e-07
DISU vs. IL7-1.281719135642832.8527656346089e-060.000311633637258907
DISU vs. SAHA0.2494222958642490.6110879795891160.877659065018429
DMSO vs. AZA-0.04783876052455010.8110321026574481
DMSO vs. CD32.031174770253761.13817533131666e-068.09948576584104e-06
DMSO vs. DISU-0.4206378021255320.094090767396690.558542532800995
DMSO vs. IL7-0.8450136117366030.0004672231840263660.0329130350429788
DMSO vs. SAHA0.6602693408157240.169673770269990.497724504430107
HIV vs. Mock in Activation0.5183051725841920.5476936734235140.999983755607037
HIV vs. Mock in Latency0.5362551590385790.002087733807102370.111952621207164
IL7 vs. CD31.193677465079650.00846122328756760.0259959271531811
SAHA vs. CD32.690820073807621.79144273132481e-050.000104680271272062
SAHA vs. IL71.516156678908610.003866056068794310.0320027068539294
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -1.25892 0.000305467
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
203657_s_at 1.52 No downregulated in CD8+ cells

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

Drugbank ID Drug Name Drug Status Pharmacological Action Drug Action
DB02243 4-Morpholin-4-Yl-Piperidine-1-Carboxylic Acid [1-(3-Benzenesulfonyl-1-Propyl-Allylcarbamoyl)-2-Phenylethyl]-Amide experimental unknown unknown
DB01810 [1-(1-Methyl-4,5-Dioxo-Pent-2-Enylcarbamoyl)-2-Phenyl-Ethyl]-Carbamic Acid Benzyl Ester experimental unknown unknown
DB07913 HOMOPHENYLALANINYLMETHANE experimental unknown unknown
DB03573 WRR-99 experimental unknown unknown
DB03691 WRR-112 experimental unknown unknown
DB01871 [1-(1-Benzyl-3-Hydroxy-2-Oxo-Propylcarbamoyl)-2-Phenyl-Ethyl]-Carbamic Acid Benzyl Ester experimental unknown unknown
DB03536 Benzoyl-Arginine-Alanine-Methyl Ketone experimental unknown unknown
DB08775 BENZOYL-TYROSINE-ALANINE-METHYL KETONE experimental unknown unknown

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
1D5U Model - A=271-484.
1M6D X-ray 1.7Å A/B=271-484.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04142 Lysosome - Homo sapiens (human)
hsa04210 Apoptosis - Homo sapiens (human)