Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0000716
UniProt IDP17812
Primary gene name(s)CTPS1
Synonym gene name(s)CTPS
Protein nameCTP synthase 1
Protein functionThis enzyme is involved in the de novo synthesis of CTP, a precursor of DNA, RNA and phospholipids. Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as a source of nitrogen. This enzyme and its product, CTP, play a crucial role in the proliferation of activated lymphocytes and therefore in immunity. {ECO:0000269|PubMed:16179339, ECO:0000269|PubMed:24870241}.
Subcellular locationunknown
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P17812
Gene Ontology
(Biological Process)
Complete annatation
'de novo' CTP biosynthetic process [GO:0044210];
B cell proliferation [GO:0042100];
CTP biosynthetic process [GO:0006241];
glutamine metabolic process [GO:0006541];
nucleobase-containing compound metabolic process [GO:0006139];
nucleobase-containing small molecule interconversion [GO:0015949];
response to drug [GO:0042493];
T cell proliferation [GO:0042098]
Gene Ontology
(Molecular Function)
Complete annatation
ATP binding [GO:0005524];
CTP synthase activity [GO:0003883]
Gene Ontology
(Cellular Component)
Complete annatation
cytosol [GO:0005829];
membrane [GO:0016020]
Protein-protein interaction107883
Phylogenetic treeP17812
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD32.357491439104997.52919948610042e-121.58270703841709e-10
AZA vs. DISU-0.007727076085997010.9761475649464120.997811953897277
AZA vs. IL70.6612045276902870.007490765964826670.275198110741028
AZA vs. SAHA-0.9016938416759270.0003217314727775870.009344470560018
DISU vs. CD3-2.378587401200774.50613435454272e-109.80165501592742e-09
DISU vs. IL70.6601526872656610.01112270649657820.125505090146644
DISU vs. SAHA-0.8924127723082010.002847843241635340.0436177291612665
DMSO vs. AZA0.09884219271077590.6867275768284351
DMSO vs. CD3-2.272078660890481.21155308008269e-112.2070190025932e-10
DMSO vs. DISU0.1042934186249470.6887346699564240.957954410639349
DMSO vs. IL70.56984675096770.02206723309756240.304080017317873
DMSO vs. SAHA-1.006557972840393.34807125409453e-050.00149729323057741
HIV vs. Mock in Activation0.0339914408159330.9563732492933570.999983755607037
HIV vs. Mock in Latency-0.3788075670073560.02436213951426260.587575825660791
IL7 vs. CD3-1.688445178153573.08728966280647e-073.38680619268427e-06
SAHA vs. CD3-3.2840454798691300
SAHA vs. IL7-1.566078566554715.74926106722273e-107.10363617437012e-08
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.602918 0.000305467
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.992 0.936 0.898 0.841 0.967
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
202613_at 1.61 No upregulated in CD8+ cells

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

Drugbank ID Drug Name Drug Status Pharmacological Action Drug Action
DB00130 L-Glutamine approved, investigational, nutraceutical unknown antagonist

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
2VO1 X-ray 2.8Å A/B=1-273.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Tat upregulates 23166591

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa00240 Pyrimidine metabolism - Homo sapiens (human)
hsa01100 Metabolic pathways - Homo sapiens (human)