Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0000704
UniProt IDP48729
Primary gene name(s)CSNK1A1
Synonym gene name(s)unknown
Protein nameCasein kinase I isoform alpha
Protein functionCasein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates CTNNB1 at 'Ser-45'. May phosphorylate PER1 and PER2. May play a role in segregating chromosomes during mitosis, PubMed:11955436, PubMed:1409656, PubMed:18305108. May play a role in keratin cytoskeleton disassembly and thereby, it may regulate epithelial cell migration, PubMed:23902688. {ECO:0000269|PubMed:11955436, ECO:0000269|PubMed:1409656, ECO:0000269|PubMed:18305108, ECO:0000269|PubMed:23902688}.
Subcellular locationCytoplasm {ECO:0000269|PubMed:1409656}. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome {ECO:0000269|PubMed:1409656}. Chromosome, centromere, kinetochore {ECO:0000269|PubMed:1409656}. Nucleus speckle {ECO:0000303|PubMed:18305108}. Note=Localizes to the centrosome in interphase cells, and to kinetochore fibers during mitosis. Also recruited to the keratin cytoskeleton, PubMed:23902688. {ECO:0000269|PubMed:1409656, ECO:0000269|PubMed:23902688}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P48729
Gene Ontology
(Biological Process)
Complete annatation
beta-catenin destruction complex assembly [GO:1904885];
beta-catenin destruction complex disassembly [GO:1904886];
cell division [GO:0051301];
cell morphogenesis [GO:0000902];
cell surface receptor signaling pathway [GO:0007166];
Golgi organization [GO:0007030];
intermediate filament cytoskeleton organization [GO:0045104];
mitotic nuclear division [GO:0007067];
negative regulation of canonical Wnt signaling pathway [GO:0090090];
peptidyl-serine phosphorylation [GO:0018105];
phagocytosis [GO:0006909];
positive regulation of proteasomal ubiquitin-dependent protein catabolic process [GO:0032436];
proteasome-mediated ubiquitin-dependent protein catabolic process [GO:0043161];
protein phosphorylation [GO:0006468];
regulation of cell shape [GO:0008360];
regulation of GTP binding [GO:1904424];
signal transduction [GO:0007165];
Wnt signaling pathway [GO:0016055]
Gene Ontology
(Molecular Function)
Complete annatation
ATP binding [GO:0005524];
kinase activity [GO:0016301];
protein kinase activity [GO:0004672];
protein serine/threonine kinase activity [GO:0004674]
Gene Ontology
(Cellular Component)
Complete annatation
beta-catenin destruction complex [GO:0030877];
centrosome [GO:0005813];
condensed chromosome kinetochore [GO:0000777];
cytosol [GO:0005829];
membrane [GO:0016020];
nuclear speck [GO:0016607];
primary cilium [GO:0072372]
Protein-protein interaction107836
Phylogenetic treeP48729
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.3503362527050860.28455997275890.401018037346624
AZA vs. DISU-0.1914741525218630.4486066095021140.918669250520452
AZA vs. IL70.02961319721643390.8773901358106750.999311006273513
AZA vs. SAHA-0.05686374922545570.8154113150169020.95304910158264
DISU vs. CD3-0.5540655947401510.1271714099174150.225209331895572
DISU vs. IL70.2120750229913870.3993546861218270.763407435051866
DISU vs. SAHA0.135535040502070.6414519721235150.889153447537328
DMSO vs. AZA-0.09406387769687840.5737910562525931
DMSO vs. CD3-0.4560566721749750.1540938570918470.242446366275579
DMSO vs. DISU0.09542486786674720.6953567066083780.958674191480009
DMSO vs. IL70.1310411306544830.4655098334150980.876252870495513
DMSO vs. SAHA0.03033687212502980.8974559996147080.975026503245809
HIV vs. Mock in Activation-0.03292779824009460.9577373512629190.999983755607037
HIV vs. Mock in Latency-0.04626697892959150.7788053915513460.999834320637052
IL7 vs. CD3-0.312727270407230.3304846746404170.469013793508615
SAHA vs. CD3-0.4323815788972550.2211432199119880.326664215259132
SAHA vs. IL7-0.09030489177460160.7104271467128520.867822190652354
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.28482 0.112158
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.953 0.9 0.878 0.782 0.88
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
5FQD X-ray 2.4Å C/F=1-337.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04310 Wnt signaling pathway - Homo sapiens (human)
hsa04340 Hedgehog signaling pathway - Homo sapiens (human)
hsa05224 Breast cancer - Homo sapiens (human)