Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0000693
UniProt IDQ9UJA2
Primary gene name(s)CRLS1
Synonym gene name(s)C20orf155, CLS1
Protein nameCardiolipin synthase
Protein functionCatalyzes the synthesis of cardiolipin, CL, diphosphatidylglycerol by specifically transferring a phosphatidyl group from CDP-diacylglycerol to phosphatidylglycerol, PG. CL is a key phospholipid in mitochondrial membranes and plays important roles in maintaining the functional integrity and dynamics of mitochondria under both optimal and stress conditions. {ECO:0000269|PubMed:16547353, ECO:0000269|PubMed:16678169}.
Subcellular locationMitochondrion inner membrane {ECO:0000269|PubMed:16547353};
Multi-pass membrane protein {ECO:0000269|PubMed:16547353}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q9UJA2
Gene Ontology
(Biological Process)
Complete annatation
cardiolipin biosynthetic process [GO:0032049];
phosphatidylglycerol acyl-chain remodeling [GO:0036148]
Gene Ontology
(Molecular Function)
Complete annatation
1-acylglycerol-3-phosphate O-acyltransferase activity [GO:0003841];
2-acylglycerol-3-phosphate O-acyltransferase activity [GO:0047144];
cardiolipin synthase activity [GO:0008808];
CDP-diacylglycerol-phosphatidylglycerol phosphatidyltransferase activity [GO:0043337]
Gene Ontology
(Cellular Component)
Complete annatation
integral component of membrane [GO:0016021];
mitochondrial inner membrane [GO:0005743];
mitochondrion [GO:0005739]
Protein-protein interaction120096
Phylogenetic treeQ9UJA2
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
      Negatively associated
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD31.242247802147170.0001891121556663040.000844532862282234
AZA vs. DISU-0.3161111098155950.2148136985544690.802304680874644
AZA vs. IL70.3195936055863060.1001079879645750.838293168105575
AZA vs. SAHA-0.2114257244390120.3899799581875680.752022245070922
DISU vs. CD3-1.570221444348742.35687050647027e-050.00015832204106298
DISU vs. IL70.6270542040214260.01363872513820230.141893568984403
DISU vs. SAHA0.1053357371532810.7191199662644870.917103213595783
DMSO vs. AZA-0.1067224681424110.5310512376276611
DMSO vs. CD3-1.359920227935252.97527659660624e-050.000152561408876354
DMSO vs. DISU0.2077787975786920.3979653519495280.862858673780775
DMSO vs. IL70.4335073015669190.01725559850674370.272554298105888
DMSO vs. SAHA-0.1118149465096840.6379106140562810.883919387366036
HIV vs. Mock in Activation-0.2970908620222010.6331542613988970.999983755607037
HIV vs. Mock in Latency-0.07383967274930260.6596803116812220.999834320637052
IL7 vs. CD3-0.9138193221346910.004801401456228540.0161832567418902
SAHA vs. CD3-1.47866901375494.1849676269301e-050.000222809950472684
SAHA vs. IL7-0.5348417878746420.0294458971922980.12887979724058
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.0446591 0.821217
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa00564 Glycerophospholipid metabolism - Homo sapiens (human)
hsa01100 Metabolic pathways - Homo sapiens (human)