Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0000667
UniProt IDQ9UNS2
Primary gene name(s)COPS3
Synonym gene name(s)CSN3
Protein nameCOP9 signalosome complex subunit 3
Protein functionComponent of the COP9 signalosome complex, CSN, a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin, Ubl conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively. {ECO:0000269|PubMed:11285227, ECO:0000269|PubMed:11337588, ECO:0000269|PubMed:12628923, ECO:0000269|PubMed:12732143, ECO:0000269|PubMed:9535219}.
Subcellular locationCytoplasm {ECO:0000269|PubMed:9535219}. Nucleus {ECO:0000269|PubMed:9535219}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q9UNS2
Gene Ontology
(Biological Process)
Complete annatation
cullin deneddylation [GO:0010388];
in utero embryonic development [GO:0001701];
nucleotide-excision repair, DNA damage recognition [GO:0000715];
response to light stimulus [GO:0009416];
signal transduction [GO:0007165];
transcription-coupled nucleotide-excision repair [GO:0006283];
ubiquitin-dependent protein catabolic process [GO:0006511]
Gene Ontology
(Molecular Function)
Complete annatation
unknown
Gene Ontology
(Cellular Component)
Complete annatation
COP9 signalosome [GO:0008180];
cytoplasm [GO:0005737];
nucleoplasm [GO:0005654];
nucleus [GO:0005634]
Protein-protein interaction114103
Phylogenetic treeQ9UNS2
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
      unknown
Brass et al., Science, 2008
      unknown
Smith et al., J. Immunol, 2010
      unknown
Interferon-stimulated
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model


DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD31.179783342240380.0003868999103451290.00158590031240734
AZA vs. DISU0.173149172998880.4960152010236480.934705997400936
AZA vs. IL70.3793627624424620.04968142278951350.651581202095615
AZA vs. SAHA-0.4112765437765630.0940018556224440.386204407645877
DISU vs. CD3-1.018953824520630.005338434501741320.017107049676711
DISU vs. IL70.1971295624098820.4348393543965040.787644578701608
DISU vs. SAHA-0.5832420578638970.04634136665765810.267692884974153
DMSO vs. AZA-0.01878558993283010.9112727765126881
DMSO vs. CD3-1.209846326221580.0001931443586535850.000805124450225861
DMSO vs. DISU-0.1937623701527440.4291410541476630.881718877024027
DMSO vs. IL70.4053333424958580.02492678759035240.321809580752529
DMSO vs. SAHA-0.3990876966380180.09231624867434520.356501812518804
HIV vs. Mock in Activation-0.1005783911628360.8714560676042690.999983755607037
HIV vs. Mock in Latency0.02271716168239680.8913854108012970.999834320637052
IL7 vs. CD3-0.7925444679522590.01413989675742830.0396080029408506
SAHA vs. CD3-1.615618819218728.00710123904924e-065.16332972496671e-05
SAHA vs. IL7-0.7937241798871650.001226267570426880.0136924128567516
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.0912548 0.600083
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.003 0.936 0.952 0.915 0.938
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
4D10 X-ray 3.8Å C/K=1-423.
4D18 X-ray 4.0Å C/K=1-423.
4WSN X-ray 5.5Å C/K/S/a/i/q=1-423.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Vpr interacts with 22190034

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
not found
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