Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0000654
UniProt IDQ9UIV1
Primary gene name(s)CNOT7
Synonym gene name(s)CAF1
Protein nameCCR4-NOT transcription complex subunit 7
Protein functionHas 3'-5' poly(A exoribonuclease activity for synthetic poly(A RNA substrate. Its function seems to be partially redundant with that of CNOT8. Catalytic component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. During miRNA-mediated repression the complex seems also to act as translational repressor during translational initiation. Additional complex functions may be a consequence of its influence on mRNA expression. Associates with members of the BTG family such as TOB1 and BTG2 and is required for their anti-proliferative activity. {ECO:0000269|PubMed:19605561, ECO:0000269|PubMed:20065043, ECO:0000269|PubMed:20634287, ECO:0000269|PubMed:23236473}.
Subcellular locationNucleus. Cytoplasm, P-body {ECO:0000250}. Note=NANOS2 promotes its localization to P-body. {ECO:0000250}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q9UIV1
Gene Ontology
(Biological Process)
Complete annatation
carbohydrate metabolic process [GO:0005975];
cytoplasmic mRNA processing body assembly [GO:0033962];
deadenylation-dependent decapping of nuclear-transcribed mRNA [GO:0000290];
DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest [GO:0006977];
exonucleolytic nuclear-transcribed mRNA catabolic process involved in deadenylation-dependent decay [GO:0043928];
gene silencing by miRNA [GO:0035195];
gene silencing by RNA [GO:0031047];
negative regulation of cell proliferation [GO:0008285];
negative regulation of gene expression [GO:0010629];
nuclear-transcribed mRNA poly(A tail shortening [GO:0000289];
positive regulation of cell proliferation [GO:0008284];
positive regulation of mRNA catabolic process [GO:0061014];
positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay [GO:1900153];
positive regulation of nuclear-transcribed mRNA poly(A tail shortening [GO:0060213];
positive regulation of transcription from RNA polymerase II promoter [GO:0045944];
signal transduction [GO:0007165];
transcription, DNA-templated [GO:0006351]
Gene Ontology
(Molecular Function)
Complete annatation
3'-5'-exoribonuclease activity [GO:0000175];
exoribonuclease activity [GO:0004532];
metal ion binding [GO:0046872];
poly(A-specific ribonuclease activity [GO:0004535];
RNA binding [GO:0003723];
signal transducer activity [GO:0004871];
transcription factor activity, sequence-specific DNA binding [GO:0003700]
Gene Ontology
(Cellular Component)
Complete annatation
CCR4-NOT complex [GO:0030014];
cytoplasmic mRNA processing body [GO:0000932];
cytosol [GO:0005829];
membrane [GO:0016020];
nucleoplasm [GO:0005654];
nucleus [GO:0005634]
Protein-protein interaction118938
Phylogenetic treeQ9UIV1
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.5242928657700140.1097559993627970.189707439823933
AZA vs. DISU-0.007246597245443390.9771329821304880.997889048290254
AZA vs. IL70.1188402024150070.5361749094732390.999311006273513
AZA vs. SAHA-0.08109419062105020.7394118351560010.927587716639611
DISU vs. CD3-0.5445270588145870.1337263217823750.234149212980314
DISU vs. IL70.1174351450587460.6408673050779260.894429670476317
DISU vs. SAHA-0.07333125506682320.8011075728965340.948428297810185
DMSO vs. AZA-0.100517743626630.5483744267519581
DMSO vs. CD3-0.6365607037485450.04708170280371280.091526379878514
DMSO vs. DISU-0.09520120697210780.6964701359430060.958803444206224
DMSO vs. IL70.2266080642674990.2075292141610780.716699329473652
DMSO vs. SAHA0.0119824469817940.9594503294500160.990606009121134
HIV vs. Mock in Activation-0.1704362233841460.7840406419414620.999983755607037
HIV vs. Mock in Latency-0.02677461261467150.8710577232477350.999834320637052
IL7 vs. CD3-0.3964555067390480.2171476693770650.343376712182176
SAHA vs. CD3-0.6312147232763020.07470751081047690.137852600454743
SAHA vs. IL7-0.204328193907390.4011524571292240.648192588777388
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
-0.3202 0.04807

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.286177 0.0317981
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.99 0.931 0.879 0.912 0.986
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
2D5R X-ray 2.5Å A=11-262.
4GMJ X-ray 2.7Å B/D/F=1-285.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa03018 RNA degradation - Homo sapiens (human)