Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0000638
UniProt IDQ9HAW4
Primary gene name(s)CLSPN
Synonym gene name(s)unknown
Protein nameClaspin
Protein functionRequired for checkpoint mediated cell cycle arrest in response to inhibition of DNA replication or to DNA damage induced by both ionizing and UV irradiation. Adapter protein which binds to BRCA1 and the checkpoint kinase CHEK1 and facilitates the ATR-dependent phosphorylation of both proteins. Can also bind specifically to branched DNA structures and may associate with S-phase chromatin following formation of the pre-replication complex, pre-RC. This may indicate a role for this protein as a sensor which monitors the integrity of DNA replication forks. {ECO:0000269|PubMed:12766152, ECO:0000269|PubMed:15096610, ECO:0000269|PubMed:15190204, ECO:0000269|PubMed:15226314, ECO:0000269|PubMed:15707391}.
Subcellular locationNucleus {ECO:0000269|PubMed:12766152}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q9HAW4
Gene Ontology
(Biological Process)
Complete annatation
activation of protein kinase activity [GO:0032147];
cellular component disassembly involved in execution phase of apoptosis [GO:0006921];
DNA damage checkpoint [GO:0000077];
DNA repair [GO:0006281];
DNA replication [GO:0006260];
G2 DNA damage checkpoint [GO:0031572];
mitotic DNA replication checkpoint [GO:0033314];
peptidyl-serine phosphorylation [GO:0018105]
Gene Ontology
(Molecular Function)
Complete annatation
anaphase-promoting complex binding [GO:0010997];
DNA binding [GO:0003677]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasm [GO:0005737];
nucleoplasm [GO:0005654]
Protein-protein interaction122015
Phylogenetic treeQ9HAW4
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD32.249687295173040.0793868890518090.145561945473098
AZA vs. DISU-0.1714333733936860.6391658310780660.960057478314726
AZA vs. IL71.101028590671850.04839510141128940.646111039479509
AZA vs. SAHA-0.6574375352837830.08761423135048860.370555991827606
DISU vs. CD3-2.433983974236750.05635043299536010.118850766331616
DISU vs. IL71.26309322074330.02354627597263850.195033027630508
DISU vs. SAHA-0.4852577479519410.2039627970035680.577696544566955
DMSO vs. AZA-0.1028005755030990.7362204494275241
DMSO vs. CD3-2.36198822242890.06846975072668560.125060232140824
DMSO vs. DISU0.06657170166165530.8251509699030330.98057019242518
DMSO vs. IL71.209697943177750.02123053476423620.298959149617488
DMSO vs. SAHA-0.5619393581446630.0810226170319690.331638202414124
HIV vs. Mock in Activation0.2579696413539710.9064873410623630.999983755607037
HIV vs. Mock in Latency0.06552477004187760.8672139709308070.999834320637052
IL7 vs. CD3-1.140753333476730.3622125936814940.499600148660428
SAHA vs. CD3-2.93377067931380.02312558749110110.0520153139538646
SAHA vs. IL7-1.762063619906130.002241940301582310.0213706325675179
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.847978 0.000305467
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.967 1.004 0.727 0.542 1.115
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
not found