Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0000630
UniProt IDQ9HAZ1
Primary gene name(s)CLK4
Synonym gene name(s)unknown
Protein nameDual specificity protein kinase CLK4
Protein functionDual specificity kinase acting on both serine/threonine and tyrosine-containing substrates. Phosphorylates serine- and arginine-rich, SR proteins of the spliceosomal complex and may be a constituent of a network of regulatory mechanisms that enable SR proteins to control RNA splicing. Phosphorylates SRSF1 and SRSF3. Required for the regulation of alternative splicing of MAPT/TAU. Regulates the alternative splicing of tissue factor, F3 pre-mRNA in endothelial cells. {ECO:0000269|PubMed:11170754, ECO:0000269|PubMed:19168442}.
Subcellular locationNucleus {ECO:0000250}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q9HAZ1
Gene Ontology
(Biological Process)
Complete annatation
protein autophosphorylation [GO:0046777];
regulation of RNA splicing [GO:0043484]
Gene Ontology
(Molecular Function)
Complete annatation
ATP binding [GO:0005524];
protein serine/threonine/tyrosine kinase activity [GO:0004712];
protein serine/threonine kinase activity [GO:0004674];
protein tyrosine kinase activity [GO:0004713]
Gene Ontology
(Cellular Component)
Complete annatation
nucleus [GO:0005634]
Protein-protein interaction121501
Phylogenetic treeQ9HAZ1
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
      Negatively associated
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.5601005211975820.08897855395360590.159957629435681
AZA vs. DISU0.2783340416857550.2915499569659150.857426660887781
AZA vs. IL7-0.2918858794392170.131342927189060.897328354403451
AZA vs. SAHA-0.381074976720580.1209799170786990.443327979107575
DISU vs. CD30.8250767337150920.02613226405828750.0639476864309458
DISU vs. IL7-0.5790879525089910.02572795121669380.204284140964739
DISU vs. SAHA-0.6588790706839550.0245728663588020.183578776071251
DMSO vs. AZA0.03571363325377740.8321481963704781
DMSO vs. CD30.5837840819502070.06914975011917620.126057718483227
DMSO vs. DISU-0.2447302236750260.334404379974680.83481441629829
DMSO vs. IL7-0.3202647657371130.07662454396245820.522676470694144
DMSO vs. SAHA-0.423968256590980.07495778257638230.320347238469702
HIV vs. Mock in Activation-0.04150883483694880.9468551218227140.999983755607037
HIV vs. Mock in Latency0.1061140775009960.5218465307602460.999834320637052
IL7 vs. CD30.2762969030396880.3908346382805690.527635513014153
SAHA vs. CD30.1534846116643510.6674048633262540.753166938020567
SAHA vs. IL7-0.09347708491455330.7036759383460090.863287303217038
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
-1.4 0.039579114 -1.6 0.004137194 -2.2 0.000419558
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.268766 0.142095
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.202 1.067 0.725 0.779 0.909
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
210346_s_at 1.36 No downregulated in CD8+ cells

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
not found