Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0000617
UniProt IDQ7Z460
Primary gene name(s)CLASP1
Synonym gene name(s)KIAA0622, MAST1
Protein nameCLIP-associating protein 1
Protein functionMicrotubule plus-end tracking protein that promotes the stabilization of dynamic microtubules. Involved in the nucleation of noncentrosomal microtubules originating from the trans-Golgi network, TGN. Required for the polarization of the cytoplasmic microtubule arrays in migrating cells towards the leading edge of the cell. May act at the cell cortex to enhance the frequency of rescue of depolymerizing microtubules by attaching their plus-ends to cortical platforms composed of ERC1 and PHLDB2. This cortical microtubule stabilizing activity is regulated at least in part by phosphatidylinositol 3-kinase signaling. Also performs a similar stabilizing function at the kinetochore which is essential for the bipolar alignment of chromosomes on the mitotic spindle. {ECO:0000269|PubMed:11290329, ECO:0000269|PubMed:12837247, ECO:0000269|PubMed:15631994, ECO:0000269|PubMed:16866869, ECO:0000269|PubMed:16914514, ECO:0000269|PubMed:17543864}.
Subcellular locationCytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, spindle. Golgi apparatus, trans-Golgi network {ECO:0000305}. Note=Localizes to microtubule plus ends. Localizes to centrosomes, kinetochores and the mitotic spindle from prometaphase. Subsequently localizes to the spindle midzone from anaphase and to the midbody from telophase. In migrating cells localizes to the plus ends of microtubules within the cell body and to the entire microtubule lattice within the lamella. Localizes to the cell cortex and this requires ERC1 and PHLDB2.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q7Z460
Gene Ontology
(Biological Process)
Complete annatation
astral microtubule organization [GO:0030953];
cell division [GO:0051301];
establishment of epithelial cell polarity [GO:0090162];
establishment of mitotic spindle localization [GO:0040001];
establishment of spindle orientation [GO:0051294];
establishment or maintenance of cell polarity [GO:0007163];
exit from mitosis [GO:0010458];
G2/M transition of mitotic cell cycle [GO:0000086];
Golgi organization [GO:0007030];
microtubule anchoring [GO:0034453];
microtubule bundle formation [GO:0001578];
microtubule cytoskeleton organization [GO:0000226];
microtubule nucleation [GO:0007020];
microtubule organizing center organization [GO:0031023];
mitotic spindle assembly [GO:0090307];
mitotic spindle organization [GO:0007052];
negative regulation of microtubule depolymerization [GO:0007026];
negative regulation of microtubule polymerization or depolymerization [GO:0031111];
negative regulation of stress fiber assembly [GO:0051497];
negative regulation of wound healing, spreading of epidermal cells [GO:1903690];
positive regulation of basement membrane assembly involved in embryonic body morphogenesis [GO:1904261];
positive regulation of epithelial cell migration [GO:0010634];
positive regulation of exocytosis [GO:0045921];
positive regulation of extracellular matrix disassembly [GO:0090091];
positive regulation of microtubule polymerization [GO:0031116];
regulation of epithelial to mesenchymal transition [GO:0010717];
regulation of focal adhesion assembly [GO:0051893];
regulation of gastrulation [GO:0010470];
regulation of microtubule cytoskeleton organization [GO:0070507];
sister chromatid cohesion [GO:0007062];
vesicle targeting [GO:0006903]
Gene Ontology
(Molecular Function)
Complete annatation
dystroglycan binding [GO:0002162];
kinetochore binding [GO:0043515];
microtubule binding [GO:0008017];
microtubule plus-end binding [GO:0051010]
Gene Ontology
(Cellular Component)
Complete annatation
basal cortex [GO:0045180];
cell cortex [GO:0005938];
centrosomal corona [GO:0031592];
centrosome [GO:0005813];
condensed chromosome kinetochore [GO:0000777];
cortical microtubule cytoskeleton [GO:0030981];
cytoplasmic microtubule [GO:0005881];
cytosol [GO:0005829];
extracellular exosome [GO:0070062];
Golgi apparatus [GO:0005794];
kinetochore [GO:0000776];
kinetochore microtubule [GO:0005828];
membrane [GO:0016020];
microtubule plus-end [GO:0035371];
spindle microtubule [GO:0005876]
Protein-protein interaction116919
Phylogenetic treeQ7Z460
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.1973882236396320.5482445701847330.660259062071458
AZA vs. DISU0.3917267764511990.1221390784068910.68130792966235
AZA vs. IL70.063555661189970.7412449813242690.999311006273513
AZA vs. SAHA0.2737633553821540.2623731245505480.634527912638371
DISU vs. CD30.5759882853074250.1132005837652340.205390659566339
DISU vs. IL7-0.3372116461815910.1809412468526270.551048342687547
DISU vs. SAHA-0.1163254670819990.690401247036450.908407519765668
DMSO vs. AZA0.04232097040810270.8009009099832671
DMSO vs. CD30.2287351780967020.4755199476449780.58598838538009
DMSO vs. DISU-0.351155511167510.1504277294998860.648069149103284
DMSO vs. IL70.02852317545788920.8740577967558160.972573168445079
DMSO vs. SAHA0.2247286008973730.3413269939540240.693841263269639
HIV vs. Mock in Activation0.3088811941567510.6211234725632430.999983755607037
HIV vs. Mock in Latency0.09281153348132220.5740914712135170.999834320637052
IL7 vs. CD30.2680464031713040.4049675813777150.54215944776284
SAHA vs. CD30.4462497310324960.2109506217980470.314661572849309
SAHA vs. IL70.2065651423366850.3973687576785490.644841926283394
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.132786 0.388892
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.019 1 1.073 1.092 1.021
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
4K92 X-ray 2.0Å A/B=284-552.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
not found