Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0000614
UniProt IDQ14008
Primary gene name(s)CKAP5
Synonym gene name(s)KIAA0097
Protein nameCytoskeleton-associated protein 5
Protein functionBinds to the plus end of microtubules and regulates microtubule dynamics and microtubule organization. Acts as processive microtubule polymerase. Promotes cytoplasmic microtubule nucleation and elongation. Plays a major role in organizing spindle poles. In spindle formation protects kinetochore microtubules from depolymerization by KIF2C and has an essential role in centrosomal microtubule assembly independently of KIF2C activity. Contributes to centrosome integrity. Acts as component of the TACC3/ch-TOG/clathrin complex proposed to contribute to stabilization of kinetochore fibers of the mitotic spindle by acting as inter-microtubule bridge. The TACC3/ch-TOG/clathrin complex is required for the maintenance of kinetochore fiber tension, PubMed:23532825. Enhances the strength of NDC80 complex-mediated kinetochore-tip microtubule attachments, PubMed:27156448. {ECO:0000269|PubMed:12569123, ECO:0000269|PubMed:18809577, ECO:0000269|PubMed:21297582, ECO:0000269|PubMed:21646404, ECO:0000269|PubMed:23532825, ECO:0000269|PubMed:27156448, ECO:0000269|PubMed:9570755}.
Subcellular locationCytoplasm, cytoskeleton, microtubule organizing center, centrosome {ECO:0000269|PubMed:21646404, ECO:0000269|PubMed:25596274, ECO:0000269|PubMed:9570755}. Cytoplasm, cytoskeleton, spindle pole {ECO:0000269|PubMed:21646404}. Cytoplasm, cytoskeleton, spindle {ECO:0000269|PubMed:21297582, ECO:0000269|PubMed:25596274, ECO:0000269|PubMed:9570755}. Chromosome, centromere, kinetochore {ECO:0000269|PubMed:25596274}. Note=Detected on centrosomes and kinetochores during interphase and mitosis independently from TACC3 and clathrin. Located to spindle poles and microtubules during mitosis. In complex with TACC3 localized to microtuble plus-ends in mitosis and interphase. In complex with TACC3 and clathrin localized to inter-microtubule bridges in mitotic spindles. Accumulation sites at microtubule plus ends protruded approximately 100 nm from MAPRE1/EB1 sites in interphase cells. {ECO:0000269|PubMed:21646404, ECO:0000269|PubMed:23251535, ECO:0000269|PubMed:25596274}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q14008
Gene Ontology
(Biological Process)
Complete annatation
cell division [GO:0051301];
centrosome duplication [GO:0051298];
centrosome organization [GO:0051297];
establishment or maintenance of microtubule cytoskeleton polarity [GO:0030951];
G2/M transition of mitotic cell cycle [GO:0000086];
microtubule polymerization [GO:0046785];
mitotic nuclear division [GO:0007067];
RNA transport [GO:0050658];
sister chromatid cohesion [GO:0007062];
spindle organization [GO:0007051]
Gene Ontology
(Molecular Function)
Complete annatation
Gene Ontology
(Cellular Component)
Complete annatation
centrosome [GO:0005813];
cytosol [GO:0005829];
membrane [GO:0016020];
protein complex [GO:0043234];
spindle pole [GO:0000922]
Protein-protein interaction115137
Phylogenetic treeQ14008
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.5379526215685620.100880458795480.177190336203878
AZA vs. DISU0.126345922480770.6173821397478180.959028958140422
AZA vs. IL70.1223794994303580.5242822062185890.999311006273513
AZA vs. SAHA0.1829347669098250.4541852804375930.795665477851764
DISU vs. CD3-0.4238543824233220.2425310622602960.368613957704346
DISU vs. IL7-0.01299674993400370.9588067127666550.99328078389483
DISU vs. SAHA0.05747614456769210.8444008667937970.959044030041097
DMSO vs. AZA0.03477625494834080.8354670632226631
DMSO vs. CD3-0.5149360876904940.1077053465161810.18156736585857
DMSO vs. DISU-0.09360592369135610.7011199149202610.958837803890706
DMSO vs. IL70.09496031152096670.5972106560839270.911970258876874
DMSO vs. SAHA0.1412110140812980.550006444541090.838558131075242
HIV vs. Mock in Activation0.1740857770989090.7799072846015160.999983755607037
HIV vs. Mock in Latency0.01861009425893960.9101954712188410.999834320637052
IL7 vs. CD3-0.4077059372390860.204511913084710.327737497566062
SAHA vs. CD3-0.3801757209841550.2834443704942330.396943282533974
SAHA vs. IL70.05685414564661750.8161314885681230.920697953800021
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.6127 0.000305467
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.997 1.106 1.361 1.447 1.064
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
4QMI X-ray 1.9Å A/B=846-1081.
4QMJ X-ray 2.5Å A=846-1081.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
integrase interacts with 24968937

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
not found