Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0000606
UniProt IDQ9Y4C5
Primary gene name(s)CHST2
Synonym gene name(s)GN6ST
Protein nameCarbohydrate sulfotransferase 2
Protein functionSulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate, PAPS as sulfonate donor to catalyze the transfer of sulfate to position 6 of non-reducing N-acetylglucosamine, GlcNAc residues within keratan-like structures on N-linked glycans and within mucin-associated glycans that can ultimately serve as SELL ligands. SELL ligands are present in high endothelial cells, HEVs and play a central role in lymphocyte homing at sites of inflammation. Participates in biosynthesis of the SELL ligand sialyl 6-sulfo Lewis X and in lymphocyte homing to Peyer patches. Has no activity toward O-linked sugars. Its substrate specificity may be influenced by its subcellular location. Sulfates GlcNAc residues at terminal, non-reducing ends of oligosaccharide chains. {ECO:0000269|PubMed:11042394}.
Subcellular locationGolgi apparatus, trans-Golgi network membrane {ECO:0000269|PubMed:12855678, ECO:0000269|PubMed:22260995};
Single-pass type II membrane protein {ECO:0000269|PubMed:12855678, ECO:0000269|PubMed:22260995}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q9Y4C5
Gene Ontology
(Biological Process)
Complete annatation
carbohydrate metabolic process [GO:0005975];
inflammatory response [GO:0006954];
keratan sulfate biosynthetic process [GO:0018146];
multicellular organism development [GO:0007275];
N-acetylglucosamine metabolic process [GO:0006044];
sulfur compound metabolic process [GO:0006790]
Gene Ontology
(Molecular Function)
Complete annatation
N-acetylglucosamine 6-O-sulfotransferase activity [GO:0001517];
sulfotransferase activity [GO:0008146]
Gene Ontology
(Cellular Component)
Complete annatation
Golgi membrane [GO:0000139];
integral component of membrane [GO:0016021];
intrinsic component of Golgi membrane [GO:0031228];
trans-Golgi network [GO:0005802]
Protein-protein interactionunknown
Phylogenetic treeQ9Y4C5
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.6516151874908440.2228616655581210.331455597325044
AZA vs. DISU-0.5074584905393220.157877391772910.740591858744402
AZA vs. IL7-0.06149348753357670.8126494647600350.999311006273513
AZA vs. SAHA0.788740958408960.002789201303012970.0422982096998164
DISU vs. CD3-1.171475514981440.04104954772987010.0921353309231778
DISU vs. IL70.4356407699379420.2025112656961450.579619770337838
DISU vs. SAHA1.299409287262610.0001934331439711330.00628282524307964
DMSO vs. AZA-0.03061374526642940.911375861919291
DMSO vs. CD3-0.6894660109885110.1975780641719970.296317923021516
DMSO vs. DISU0.4761326606765060.1793485518950580.691330415689155
DMSO vs. IL7-0.02386276986191980.9243207685664910.984376477116347
DMSO vs. SAHA0.8141322376816640.001322764412353350.0218350300751121
HIV vs. Mock in Activation0.1988727608286590.8584134118026930.999983755607037
HIV vs. Mock in Latency-0.3318843730138560.2941374789636060.999834320637052
IL7 vs. CD3-0.7091514614438630.176450697330150.2933643680628
SAHA vs. CD30.114745119312840.8344596307722420.883469624542482
SAHA vs. IL70.8493570586437550.0008691961880047390.010499160466876
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.914297 0.000305467
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Envelope surface glycoprotein gp160; precursor processed by 1433500

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa00533 Glycosaminoglycan biosynthesis - keratan sulfate - Homo sapiens (human)