Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0000590
UniProt IDP13569
Primary gene name(s)CFTR
Synonym gene name(s)ABCC7
Protein nameCystic fibrosis transmembrane conductance regulator
Protein functionInvolved in the transport of chloride ions. May regulate bicarbonate secretion and salvage in epithelial cells by regulating the SLC4A7 transporter. Can inhibit the chloride channel activity of ANO1. Plays a role in the chloride and bicarbonate homeostasis during sperm epididymal maturation and capacitation. {ECO:0000269|PubMed:22178883}.
Subcellular locationEarly endosome membrane {ECO:0000269|PubMed:19398555};
Multi-pass membrane protein {ECO:0000255}. Cell membrane {ECO:0000269|PubMed:22178883};
Multi-pass membrane protein {ECO:0000255}. Note=In the oviduct and bronchus, detected on the apical side of epithelial cells, but not associated with cilia. {ECO:0000269|PubMed:22207244}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P13569
Gene Ontology
(Biological Process)
Complete annatation
cellular response to cAMP [GO:0071320];
chloride transmembrane transport [GO:1902476];
cholesterol biosynthetic process [GO:0006695];
cholesterol transport [GO:0030301];
intracellular pH elevation [GO:0051454];
membrane hyperpolarization [GO:0060081];
positive regulation of cyclic nucleotide-gated ion channel activity [GO:1902161];
positive regulation of exocytosis [GO:0045921];
positive regulation of insulin secretion involved in cellular response to glucose stimulus [GO:0035774];
positive regulation of voltage-gated chloride channel activity [GO:1902943];
respiratory gaseous exchange [GO:0007585];
sperm capacitation [GO:0048240];
transmembrane transport [GO:0055085];
transport [GO:0006810];
vesicle docking involved in exocytosis [GO:0006904]
Gene Ontology
(Molecular Function)
Complete annatation
anion transmembrane-transporting ATPase activity [GO:0043225];
ATP binding [GO:0005524];
ATP-binding and phosphorylation-dependent chloride channel activity [GO:0005224];
bicarbonate transmembrane transporter activity [GO:0015106];
channel-conductance-controlling ATPase activity [GO:0005260];
chloride channel activity [GO:0005254];
chloride channel inhibitor activity [GO:0019869];
chloride channel regulator activity [GO:0017081];
chloride transmembrane transporter activity [GO:0015108];
enzyme binding [GO:0019899];
PDZ domain binding [GO:0030165]
Gene Ontology
(Cellular Component)
Complete annatation
apical plasma membrane [GO:0016324];
basolateral plasma membrane [GO:0016323];
cell surface [GO:0009986];
chloride channel complex [GO:0034707];
cytoplasm [GO:0005737];
cytosol [GO:0005829];
early endosome [GO:0005769];
early endosome membrane [GO:0031901];
extracellular exosome [GO:0070062];
Golgi-associated vesicle membrane [GO:0030660];
lysosomal membrane [GO:0005765];
plasma membrane [GO:0005886];
protein complex [GO:0043234];
recycling endosome [GO:0055037]
Protein-protein interaction107506
Phylogenetic treeP13569
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3unknownunknownunknown
AZA vs. DISUunknownunknownunknown
AZA vs. IL7unknownunknownunknown
AZA vs. SAHAunknownunknownunknown
DISU vs. CD3unknownunknownunknown
DISU vs. IL7unknownunknownunknown
DISU vs. SAHAunknownunknownunknown
DMSO vs. AZAunknownunknownunknown
DMSO vs. CD3unknownunknownunknown
DMSO vs. DISUunknownunknownunknown
DMSO vs. IL7unknownunknownunknown
DMSO vs. SAHAunknownunknownunknown
HIV vs. Mock in Activationunknownunknownunknown
HIV vs. Mock in Latencyunknownunknownunknown
IL7 vs. CD3unknownunknownunknown
SAHA vs. CD3unknownunknownunknown
SAHA vs. IL7unknownunknownunknown
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
NOTEST 2.68752 1
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

Drugbank ID Drug Name Drug Status Pharmacological Action Drug Action
DB00887 Bumetanide approved unknown antagonist
DB01016 Glyburide approved unknown antagonist
DB04941 Crofelemer approved yes antagonist
DB04395 Phosphoaminophosphonic Acid-Adenylate Ester experimental unknown unknown
DB04522 Phosphonoserine experimental unknown unknown
DB08820 Ivacaftor approved yes potentiator
DB01050 Ibuprofen approved unknown inhibitor
DB09280 Lumacaftor approved unknown modulator

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
1NBD Model - A=425-638.
1XMI X-ray 2.2Å A/B/C/D/E=389-678.
1XMJ X-ray 2.3Å A=389-677.
2BBO X-ray 2.5Å A=389-678.
2BBS X-ray 2.0Å A/B=389-677.
2BBT X-ray 2.3Å A/B=389-678.
2LOB NMR - B=1473-1480.
2PZE X-ray 1.7Å A/B=387-646.
2PZF X-ray 2.0Å A/B=387-646.
2PZG X-ray 1.8Å A/B=375-646.
3GD7 X-ray 2.7Å A/B/C/D=1193-1427.
3ISW X-ray 2.8Å C=5-20.
4WZ6 X-ray 2.0Å A=389-678.
5D2D X-ray 2.1Å C=747-774.
5D3E X-ray 2.7Å C/G/K=762-801.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa02010 ABC transporters - Homo sapiens (human)
hsa04024 cAMP signaling pathway - Homo sapiens (human)
hsa04152 AMPK signaling pathway - Homo sapiens (human)
hsa04530 Tight junction - Homo sapiens (human)
hsa04971 Gastric acid secretion - Homo sapiens (human)
hsa04972 Pancreatic secretion - Homo sapiens (human)
hsa04976 Bile secretion - Homo sapiens (human)
hsa05110 Vibrio cholerae infection - Homo sapiens (human)