Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0000582
UniProt IDP49454
Primary gene name(s)CENPF
Synonym gene name(s)unknown
Protein nameCentromere protein F
Protein functionRequired for kinetochore function and chromosome segregation in mitosis. Required for kinetochore localization of dynein, LIS1, NDE1 and NDEL1. Regulates recycling of the plasma membrane by acting as a link between recycling vesicles and the microtubule network though its association with STX4 and SNAP25. Acts as a potential inhibitor of pocket protein-mediated cellular processes during development by regulating the activity of RB proteins during cell division and proliferation. May play a regulatory or permissive role in the normal embryonic cardiomyocyte cell cycle and in promoting continued mitosis in transformed, abnormally dividing neonatal cardiomyocytes. Interaction with RB directs embryonic stem cells toward a cardiac lineage. Involved in the regulation of DNA synthesis and hence cell cycle progression, via its C-terminus. Has a potential role regulating skeletal myogenesis and in cell differentiation in embryogenesis. Involved in dendritic cell regulation of T-cell immunity against chlamydia. {ECO:0000269|PubMed:12974617, ECO:0000269|PubMed:17600710, ECO:0000269|PubMed:7542657, ECO:0000269|PubMed:7651420}.
Subcellular locationCytoplasm, perinuclear region. Nucleus matrix. Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, spindle. Note=Relocalizes to the kinetochore/centromere, coronal surface of the outer plate and the spindle during mitosis. Observed in nucleus during interphase but not in the nucleolus. At metaphase becomes localized to areas including kinetochore and mitotic apparatus as well as cytoplasm. By telophase, is concentrated within the intracellular bridge at either side of the mid-body.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P49454
Gene Ontology
(Biological Process)
Complete annatation
cell differentiation [GO:0030154];
cell division [GO:0051301];
cell proliferation [GO:0008283];
chromosome segregation [GO:0007059];
DNA biosynthetic process [GO:0071897];
kidney development [GO:0001822];
kinetochore assembly [GO:0051382];
metaphase plate congression [GO:0051310];
mitotic cell cycle [GO:0000278];
mitotic nuclear division [GO:0007067];
mitotic spindle assembly checkpoint [GO:0007094];
muscle organ development [GO:0007517];
negative regulation of transcription, DNA-templated [GO:0045892];
protein transport [GO:0015031];
regulation of cell cycle [GO:0051726];
regulation of G2/M transition of mitotic cell cycle [GO:0010389];
regulation of striated muscle tissue development [GO:0016202];
response to drug [GO:0042493];
sister chromatid cohesion [GO:0007062];
ventricular system development [GO:0021591]
Gene Ontology
(Molecular Function)
Complete annatation
chromatin binding [GO:0003682];
dynein binding [GO:0045502];
protein C-terminus binding [GO:0008022];
protein homodimerization activity [GO:0042803];
transcription factor binding [GO:0008134]
Gene Ontology
(Cellular Component)
Complete annatation
axoneme [GO:0005930];
centrosome [GO:0005813];
chromosome, centromeric region [GO:0000775];
ciliary basal body [GO:0036064];
ciliary transition fiber [GO:0097539];
condensed chromosome outer kinetochore [GO:0000940];
cytoplasm [GO:0005737];
cytosol [GO:0005829];
kinetochore [GO:0000776];
midbody [GO:0030496];
nuclear envelope [GO:0005635];
nuclear matrix [GO:0016363];
nucleoplasm [GO:0005654];
nucleus [GO:0005634];
perinuclear region of cytoplasm [GO:0048471];
pronucleus [GO:0045120];
spindle [GO:0005819];
spindle pole [GO:0000922]
Protein-protein interaction107492
Phylogenetic treeP49454
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.5954248854664680.3972935440507310.518620106703626
AZA vs. DISU-0.06755990113304780.8415850206944990.986881421797199
AZA vs. IL70.01015682509024590.9798262204810930.999311006273513
AZA vs. SAHA-0.1966375248055050.5394869973221570.843006174526557
DISU vs. CD30.5158985305598210.4492865013182920.579791517924145
DISU vs. IL70.06874649085232180.8530674138870110.970688298887122
DISU vs. SAHA-0.1300085356042360.6576535039174890.895024016393397
DMSO vs. AZA-0.06314282429998370.8369016575518811
DMSO vs. CD30.5197664912408940.442392324513450.554878059178679
DMSO vs. DISU0.001762413051803990.9946318465107880.998740800034487
DMSO vs. IL70.08084815023599390.8133534599624480.962538241700332
DMSO vs. SAHA-0.1423266778681090.5514327299259690.838941200974091
HIV vs. Mock in Activation0.3466044966018450.8035805299406640.999983755607037
HIV vs. Mock in Latency0.2376484019172690.5415667965439010.999834320637052
IL7 vs. CD30.615282496556130.3897358972495030.526623861877343
SAHA vs. CD30.3711984930162880.5959283328582010.694236238108619
SAHA vs. IL7-0.2118607649237220.5533608852322480.76554350073338
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
1.9 0.002078971 1.2 0.422482408 1.4 0.269009104
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.275506 0.0399754
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.223 1.497 1.805 1.296 1.209
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
207828_s_at 3.86 No upregulated in CD8+ cells
209172_s_at 2.41 No upregulated in CD8+ cells

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
not found