Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0000551
UniProt IDP30305
Primary gene name(s)CDC25B
Synonym gene name(s)CDC25HU2
Protein nameM-phase inducer phosphatase 2
Protein functionTyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Directly dephosphorylates CDK1 and stimulates its kinase activity. The three isoforms seem to have a different level of activity. {ECO:0000269|PubMed:17332740}.
Subcellular locationCytoplasm, cytoskeleton, microtubule organizing center, centrosome {ECO:0000269|PubMed:15128871, ECO:0000269|PubMed:15311285, ECO:0000269|PubMed:15908796}. Cytoplasm, cytoskeleton, spindle pole {ECO:0000269|PubMed:15908796}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P30305
Gene Ontology
(Biological Process)
Complete annatation
cell division [GO:0051301];
female meiosis I [GO:0007144];
G2/M transition of mitotic cell cycle [GO:0000086];
mitotic cell cycle [GO:0000278];
mitotic nuclear division [GO:0007067];
oocyte maturation [GO:0001556];
positive regulation of cell proliferation [GO:0008284];
positive regulation of cytokinesis [GO:0032467];
positive regulation of mitotic cell cycle [GO:0045931];
positive regulation of protein kinase activity [GO:0045860];
protein phosphorylation [GO:0006468]
Gene Ontology
(Molecular Function)
Complete annatation
phosphoprotein phosphatase activity [GO:0004721];
protein kinase binding [GO:0019901];
protein tyrosine phosphatase activity [GO:0004725]
Gene Ontology
(Cellular Component)
Complete annatation
centrosome [GO:0005813];
cytoplasm [GO:0005737];
cytosol [GO:0005829];
nucleoplasm [GO:0005654];
spindle pole [GO:0000922]
Protein-protein interaction107429
Phylogenetic treeP30305
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-2.264162796934853.98123756184532e-117.50065156651658e-10
AZA vs. DISU-0.03304244724386310.8958200520734430.993937521279788
AZA vs. IL7-0.2282583770097650.2340167669666370.990920320206469
AZA vs. SAHA-1.103300724735376.02169340463421e-050.00266190634549725
DISU vs. CD32.217796626868434.72768824089087e-097.98042245724401e-08
DISU vs. IL7-0.2039526849708790.4173570329236550.775971854832129
DISU vs. SAHA-1.068660198835060.0003515180724724680.00970264243269483
DMSO vs. AZA0.1338214962445550.4226380560798361
DMSO vs. CD32.380722512588821.38677958005928e-122.95918333903585e-11
DMSO vs. DISU0.1634946450066370.5018937787880340.909029253390201
DMSO vs. IL7-0.3539847166278860.0485251513374280.438822229401156
DMSO vs. SAHA-1.24180478471091.44079650133966e-050.000753966808044791
HIV vs. Mock in Activation0.1849692634077370.7664797585195790.999983755607037
HIV vs. Mock in Latency-0.02564887903804230.926442407338080.999834320637052
IL7 vs. CD32.043557990576528.8225582395296e-101.59834639837055e-08
SAHA vs. CD31.13684239903340.001605914513346910.00537206031434803
SAHA vs. IL7-0.8790525453452970.001047898436166590.012058031318149
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.0592203 0.740832
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.049 1.267 1.585 1.988 1.028
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

Drugbank ID Drug Name Drug Status Pharmacological Action Drug Action
DB03661 Cysteinesulfonic Acid experimental unknown unknown

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
1CWR X-ray 2.1Å A=370-580.
1CWS X-ray 2.0Å A=370-580.
1CWT X-ray 2.3Å A=388-565.
1QB0 X-ray 1.9Å A=370-580.
1YM9 X-ray 2.0Å A=391-564.
1YMD X-ray 1.7Å A=391-564.
1YMK X-ray 1.7Å A=391-564.
1YML X-ray 1.7Å A=391-564.
1YS0 X-ray 2.0Å A=391-564.
2A2K X-ray 1.5Å A=391-564.
2IFD X-ray 2.0Å A=391-564.
2IFV X-ray 1.6Å A=391-564.
2UZQ X-ray 2.3Å A/B/C/D/E/F=391-580.
3FQT X-ray 1.8Å C=38-46.
3FQU X-ray 1.8Å C=38-46.
4WH7 X-ray 1.6Å A=386-565.
4WH9 X-ray 1.5Å A=386-565.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Vpr relocalizes 18060429

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04010 MAPK signaling pathway - Homo sapiens (human)
hsa04110 Cell cycle - Homo sapiens (human)
hsa04914 Progesterone-mediated oocyte maturation - Homo sapiens (human)
hsa05206 MicroRNAs in cancer - Homo sapiens (human)