Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0000548
UniProt IDQ12834
Primary gene name(s)CDC20
Synonym gene name(s)unknown
Protein nameCell division cycle protein 20 homolog
Protein functionRequired for full ubiquitin ligase activity of the anaphase promoting complex/cyclosome, APC/C and may confer substrate specificity upon the complex. Is regulated by MAD2L1: in metaphase the MAD2L1-CDC20-APC/C ternary complex is inactive and in anaphase the CDC20-APC/C binary complex is active in degrading substrates. The CDC20-APC/C complex positively regulates the formation of synaptic vesicle clustering at active zone to the presynaptic membrane in postmitotic neurons. CDC20-APC/C-induced degradation of NEUROD2 induces presynaptic differentiation. {ECO:0000269|PubMed:9637688, ECO:0000269|PubMed:9734353, ECO:0000269|PubMed:9811605}.
Subcellular locationCytoplasm, cytoskeleton, microtubule organizing center, centrosome {ECO:0000269|PubMed:20034488}. Cytoplasm, cytoskeleton, spindle pole {ECO:0000269|PubMed:20034488}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q12834
Gene Ontology
(Biological Process)
Complete annatation
anaphase-promoting complex-dependent catabolic process [GO:0031145];
cell cycle [GO:0007049];
cell division [GO:0051301];
mitotic nuclear division [GO:0007067];
negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle [GO:0051436];
positive regulation of cell proliferation [GO:0008284];
positive regulation of synapse maturation [GO:0090129];
positive regulation of synaptic plasticity [GO:0031915];
positive regulation of ubiquitin protein ligase activity [GO:1904668];
positive regulation of ubiquitin-protein ligase activity involved in regulation of mitotic cell cycle transition [GO:0051437];
proteasome-mediated ubiquitin-dependent protein catabolic process [GO:0043161];
protein ubiquitination involved in ubiquitin-dependent protein catabolic process [GO:0042787];
regulation of dendrite development [GO:0050773];
regulation of meiotic nuclear division [GO:0040020];
regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle [GO:0051439];
sister chromatid cohesion [GO:0007062]
Gene Ontology
(Molecular Function)
Complete annatation
enzyme binding [GO:0019899];
protein C-terminus binding [GO:0008022];
ubiquitin-protein transferase activator activity [GO:0097027]
Gene Ontology
(Cellular Component)
Complete annatation
centrosome [GO:0005813];
cytoplasm [GO:0005737];
cytosol [GO:0005829];
nucleoplasm [GO:0005654];
nucleus [GO:0005634];
perinuclear region of cytoplasm [GO:0048471];
protein complex [GO:0043234];
spindle [GO:0005819];
spindle pole [GO:0000922]
Protein-protein interaction107427
Phylogenetic treeQ12834
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD31.21822130639640.1447135000733530.235926171820706
AZA vs. DISU-0.4678922336268290.5644840686478810.951925020821155
AZA vs. IL7-0.08565777127432260.9097350328470320.999311006273513
AZA vs. SAHA-1.074353693509880.1857266902427780.550557437423883
DISU vs. CD3-1.698173235917140.03974021119818260.089730968184752
DISU vs. IL70.3723577623256160.6113903712503190.880844111640685
DISU vs. SAHA-0.6057845899246650.439535759171990.784923101324719
DMSO vs. AZA-0.07914268171497760.922248563216961
DMSO vs. CD3-1.314965919361290.1052734714177220.178236103271295
DMSO vs. DISU0.3844903135578880.625322622625180.944878155252929
DMSO vs. IL70.002142965566282220.9976541871136270.999784441946487
DMSO vs. SAHA-1.000460311331630.202371122004420.542693092478401
HIV vs. Mock in Activation0.6845954470213250.6238485623459450.999983755607037
HIV vs. Mock in Latency0.1505291422630040.793555729679680.999834320637052
IL7 vs. CD3-1.297123812037120.09037722377785620.175097158612291
SAHA vs. CD3-2.319497970658180.005502541763679150.0155052282221081
SAHA vs. IL7-0.991559140101090.1729823475123210.398097084946677
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.442723 0.000305467
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.995 1.371 1.289 1.137 0.878
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
4GGA X-ray 2.0Å A=81-499.
4GGC X-ray 1.3Å A=161-477.
4GGD X-ray 2.4Å A/B=71-499.
4N14 X-ray 2.1Å A=165-477.
5G04 EM 4.0Å R=1-499.
5KHR EM 6.1Å R=1-499.
5LCW EM 4.0Å Q=126-499# R=1-499.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Tat modulates 24742347
Tat downregulates 16751065

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04110 Cell cycle - Homo sapiens (human)
hsa04114 Oocyte meiosis - Homo sapiens (human)
hsa04120 Ubiquitin mediated proteolysis - Homo sapiens (human)
hsa05166 HTLV-I infection - Homo sapiens (human)
hsa05203 Viral carcinogenesis - Homo sapiens (human)