Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0000544
UniProt IDP21926
Primary gene name(s)CD9
Synonym gene name(s)MIC3, TSPAN29
Protein nameCD9 antigen
Protein functionInvolved in platelet activation and aggregation. Regulates paranodal junction formation. Involved in cell adhesion, cell motility and tumor metastasis. Required for sperm-egg fusion. {ECO:0000269|PubMed:14575715, ECO:0000269|PubMed:7511626, ECO:0000269|PubMed:8478605}.
Subcellular locationMembrane {ECO:0000269|PubMed:19640571};
Multi-pass membrane protein {ECO:0000269|PubMed:19640571}. Cell membrane {ECO:0000269|PubMed:19640571};
Multi-pass membrane protein {ECO:0000269|PubMed:19640571}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P21926
Gene Ontology
(Biological Process)
Complete annatation
brain development [GO:0007420];
cell adhesion [GO:0007155];
cell surface receptor signaling pathway [GO:0007166];
cellular response to low-density lipoprotein particle stimulus [GO:0071404];
fusion of sperm to egg plasma membrane [GO:0007342];
movement of cell or subcellular component [GO:0006928];
negative regulation of cell proliferation [GO:0008285];
oligodendrocyte development [GO:0014003];
paranodal junction assembly [GO:0030913];
platelet activation [GO:0030168];
platelet degranulation [GO:0002576];
receptor internalization [GO:0031623];
response to water deprivation [GO:0009414]
Gene Ontology
(Molecular Function)
Complete annatation
unknown
Gene Ontology
(Cellular Component)
Complete annatation
apical plasma membrane [GO:0016324];
clathrin-coated endocytic vesicle membrane [GO:0030669];
endocytic vesicle membrane [GO:0030666];
external side of plasma membrane [GO:0009897];
extracellular exosome [GO:0070062];
extracellular space [GO:0005615];
extracellular vesicle [GO:1903561];
focal adhesion [GO:0005925];
integral component of plasma membrane [GO:0005887];
membrane [GO:0016020];
plasma membrane [GO:0005886];
platelet alpha granule membrane [GO:0031092]
Protein-protein interaction107366
Phylogenetic treeP21926
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
      unknown
Brass et al., Science, 2008
      unknown
Smith et al., J. Immunol, 2010
      unknown
Interferon-stimulated
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model


DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD31.482769902636810.0001617652459565690.000737708256295735
AZA vs. DISU0.2879910340654190.290016068468910.85676070959683
AZA vs. IL7-0.5268088281810540.0566824942064890.690210880962922
AZA vs. SAHA0.2871587580856890.2776933546911150.651246360611311
DISU vs. CD3-1.208110288655620.002656171900170310.00954448992208994
DISU vs. IL7-0.8279100442520920.006163763582884060.0855605303977691
DISU vs. SAHA-0.0006981198749838820.998192715400130.999572425883731
DMSO vs. AZA-0.06783708923674860.7301806111729151
DMSO vs. CD3-1.561187188141782.12002200751238e-050.000113015327470538
DMSO vs. DISU-0.3574297134608780.1702712710880080.681340636893293
DMSO vs. IL7-0.4535488058772630.05956522883088480.470727250245181
DMSO vs. SAHA0.3475402336045580.1720897636977870.502142270801422
HIV vs. Mock in Activation0.1286970581748480.8706681480246480.999983755607037
HIV vs. Mock in Latency-0.2989888096709690.4478855570789460.999834320637052
IL7 vs. CD3-2.00800898062684.55023184298131e-063.80608460428731e-05
SAHA vs. CD3-1.222564136164760.00380782043167260.0112849270216925
SAHA vs. IL70.8091940356314490.01167415238139280.0696383747515295
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
2.8 0.000530191 2 0.004655261 2.5 0.063214926
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.701861 0.000305467
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
201005_at 2.99 No downregulated in CD8+ cells

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

Drugbank ID Drug Name Drug Status Pharmacological Action Drug Action
DB05398 C31G investigational unknown unknown

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Pr55(Gag) recruits 20727121
21813604
24991932
matrix co-localizes with 24991932
Pr55(Gag) co-localizes with 16735575
16940516
18034776
18321974
19426143
19458002
19802344
20727121
22980332
24145510
25009540
Envelope surface glycoprotein gp120 inhibited by 17015697
1798917317989173
18725936
19458002
19894777
2460808519458002
19602278
19894777
Pr55(Gag) interacts with 19458002

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04640 Hematopoietic cell lineage - Homo sapiens (human)
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