Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0000542
UniProt IDP10966
Primary gene name(s)CD8B
Synonym gene name(s)CD8B1
Protein nameT-cell surface glycoprotein CD8 beta chain
Protein functionIdentifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing.
Subcellular locationIsoform 1: Cell membrane {ECO:0000305};
Single-pass type I membrane protein {ECO:0000305}.;
SUBCELLULAR LOCATION: Isoform 2: Cell membrane {ECO:0000305};
Single-pass type I membrane protein {ECO:0000305}.;
SUBCELLULAR LOCATION: Isoform 3: Secreted {ECO:0000305}.;
SUBCELLULAR LOCATION: Isoform 4: Cell membrane {ECO:0000305};
Single-pass type I membrane protein {ECO:0000305}.;
SUBCELLULAR LOCATION: Isoform 5: Cell membrane {ECO:0000305};
Single-pass type I membrane protein {ECO:0000305}.;
SUBCELLULAR LOCATION: Isoform 6: Secreted {ECO:0000305}.;
SUBCELLULAR LOCATION: Isoform 7: Secreted {ECO:0000305}.;
SUBCELLULAR LOCATION: Isoform 8: Secreted {ECO:0000305}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P10966
Gene Ontology
(Biological Process)
Complete annatation
adaptive immune response [GO:0002250];
immune response [GO:0006955];
regulation of defense response to virus by virus [GO:0050690];
regulation of immune response [GO:0050776];
T cell activation [GO:0042110];
transmembrane receptor protein tyrosine kinase signaling pathway [GO:0007169]
Gene Ontology
(Molecular Function)
Complete annatation
coreceptor activity [GO:0015026];
MHC class I protein binding [GO:0042288]
Gene Ontology
(Cellular Component)
Complete annatation
early endosome membrane [GO:0031901];
external side of plasma membrane [GO:0009897];
extracellular region [GO:0005576];
integral component of plasma membrane [GO:0005887];
plasma membrane [GO:0005886];
T cell receptor complex [GO:0042101]
Protein-protein interaction107364
Phylogenetic treeP10966
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
      unknown
Brass et al., Science, 2008
      unknown
Smith et al., J. Immunol, 2010
      unknown
Interferon-stimulated
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model


DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3unknownunknownunknown
AZA vs. DISU-0.1512237425285220.6274056381393250.960057478314726
AZA vs. IL70.3123589162212190.2291393771184120.989349135292416
AZA vs. SAHAunknownunknownunknown
DISU vs. CD3unknownunknownunknown
DISU vs. IL70.4523935725905090.133812935997140.478838663221854
DISU vs. SAHAunknownunknownunknown
DMSO vs. AZA0.1052930836987940.6721825301607851
DMSO vs. CD3unknownunknownunknown
DMSO vs. DISU0.2543594816616320.3929552516604550.861526879927911
DMSO vs. IL70.2147930777363520.3751357358844410.831519022414553
DMSO vs. SAHA-0.4630883419421180.1228323244849940.41835162990132
HIV vs. Mock in Activationunknownunknownunknown
HIV vs. Mock in Latencyunknownunknownunknown
IL7 vs. CD31.422827601352670.006597621986298210.0211452171378849
SAHA vs. CD3unknownunknownunknown
SAHA vs. IL7-0.6663609232931680.02856515203474490.126299970224801
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
-1.024 0.02022

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
2.1 0.001355917 1.6 0.027077884 1.6 0.010562123
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.62207 0.614849
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Nef co-localizes with 16103193
Nef downregulates 16103193
18473783
19149577
21543478
21922073
22013062
22844345
2449536216103193
1914957721543478
Envelope surface glycoprotein gp160; precursor inhibits 21936714
Pol inhibits 21936714

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04514 Cell adhesion molecules (CAMs) - Homo sapiens (human)
hsa04612 Antigen processing and presentation - Homo sapiens (human)
hsa04640 Hematopoietic cell lineage - Homo sapiens (human)
hsa04660 T cell receptor signaling pathway - Homo sapiens (human)
hsa05340 Primary immunodeficiency - Homo sapiens (human)
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