Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0000531
UniProt IDP07766
Primary gene name(s)CD3E
Synonym gene name(s)T3E
Protein nameT-cell surface glycoprotein CD3 epsilon chain
Protein functionThe CD3 complex mediates signal transduction, resulting in T-cell activation and proliferation. Required for normal immune responses, PubMed:15546002, PubMed:8490660. {ECO:0000269|PubMed:15546002, ECO:0000269|PubMed:8490660, ECO:0000305|PubMed:15294938}.
Subcellular locationCell membrane {ECO:0000269|PubMed:15294938};
Single-pass type I membrane protein {ECO:0000305}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P07766
Gene Ontology
(Biological Process)
Complete annatation
apoptotic signaling pathway [GO:0097190];
cell surface receptor signaling pathway [GO:0007166];
G-protein coupled receptor signaling pathway [GO:0007186];
negative regulation of gene expression [GO:0010629];
negative regulation of smoothened signaling pathway [GO:0045879];
negative thymic T cell selection [GO:0045060];
positive regulation of alpha-beta T cell proliferation [GO:0046641];
positive regulation of calcium-mediated signaling [GO:0050850];
positive regulation of gene expression [GO:0010628];
positive regulation of interferon-gamma production [GO:0032729];
positive regulation of interleukin-2 biosynthetic process [GO:0045086];
positive regulation of interleukin-4 production [GO:0032753];
positive regulation of peptidyl-tyrosine phosphorylation [GO:0050731];
positive regulation of T cell anergy [GO:0002669];
positive regulation of T cell proliferation [GO:0042102];
protein complex assembly [GO:0006461];
regulation of apoptotic process [GO:0042981];
regulation of immune response [GO:0050776];
response to nutrient [GO:0007584];
signal complex assembly [GO:0007172];
T cell activation [GO:0042110];
T cell costimulation [GO:0031295];
T cell receptor signaling pathway [GO:0050852];
transmembrane receptor protein tyrosine kinase signaling pathway [GO:0007169]
Gene Ontology
(Molecular Function)
Complete annatation
protein heterodimerization activity [GO:0046982];
protein kinase binding [GO:0019901];
receptor signaling complex scaffold activity [GO:0030159];
receptor signaling protein activity [GO:0005057];
SH3 domain binding [GO:0017124];
T cell receptor binding [GO:0042608];
transmembrane signaling receptor activity [GO:0004888]
Gene Ontology
(Cellular Component)
Complete annatation
alpha-beta T cell receptor complex [GO:0042105];
cell-cell junction [GO:0005911];
external side of plasma membrane [GO:0009897];
immunological synapse [GO:0001772];
integral component of plasma membrane [GO:0005887];
plasma membrane [GO:0005886];
T cell receptor complex [GO:0042101]
Protein-protein interaction107354
Phylogenetic treeP07766
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
      unknown
Brass et al., Science, 2008
      unknown
Smith et al., J. Immunol, 2010
      unknown
Interferon-stimulated
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model


DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-1.359148863528594.44094879140655e-050.000235268823142635
AZA vs. DISU-0.3318389180990330.1888073428139210.77427300617424
AZA vs. IL7-0.139613916286950.4661342226657870.999311006273513
AZA vs. SAHA-0.7935808613655950.006858276959758820.0759043075560973
DISU vs. CD31.016768214807240.005406801489397540.0172893775246454
DISU vs. IL70.1818958353371910.4692272206978490.807386182234174
DISU vs. SAHA-0.4592974136398980.155461268521510.507682942120841
DMSO vs. AZA-0.04076548056213970.8316863707814431
DMSO vs. CD31.310753389032545.35984411665913e-050.000257974605806613
DMSO vs. DISU0.2901705702631130.2333916585460630.749302820133555
DMSO vs. IL7-0.09202197926097270.6109761879798570.915237658574009
DMSO vs. SAHA-0.7587655667750110.00913689957782660.0850562607748482
HIV vs. Mock in Activation0.1397735176748720.8220285461684270.999983755607037
HIV vs. Mock in Latency0.1622352042002540.3236263205931460.999834320637052
IL7 vs. CD31.224429982465820.0001667130200708740.000910128703921872
SAHA vs. CD30.5433206086763390.1267132814966930.210652967694999
SAHA vs. IL7-0.6555261715523550.01941204112663890.0984911167764908
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.751419 0.000305467
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.996 1.076 1.073 1.008 1.271
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

Drugbank ID Drug Name Drug Status Pharmacological Action Drug Action
DB00075 Muromonab approved, investigational yes binder

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
1A81 X-ray 3.0Å B/D/F/H/J/L=186-203.
1SY6 X-ray 2.1Å A=21-118.
1XIW X-ray 1.9Å A/E=23-126.
2ROL NMR - B=181-192.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Vpu interacts with 20179761
Vpr antagonizes 25980612
Envelope surface glycoprotein gp120 stimulates 7506554
8125527
8145026
Nef interacts with 18555888
20179761
Envelope surface glycoprotein gp120 interacts with 7539755
9263011
Envelope transmembrane glycoprotein gp41 co-localizes with 20824090
capsid inhibits 7517794
Envelope transmembrane glycoprotein gp41 inhibits 1832084
Nef antagonizes 25980612
Pr55(Gag) interacts with 25467409
8809126
9743208
Tat interacts with 9045614
9510190
17001685
Envelope surface glycoprotein gp120 inhibits 1979339
7774645
capsid interacts with 1385321
2111780
7517794
8809126
9743208
107223708809126
9743208
Vpu antagonizes 25980612
matrix co-localizes with 25467409
Pr55(Gag) inhibits 1385321
2111780
7517794
8809126
9743208
107223707517794
Pr55(Gag) regulated by 22911005
Nef downregulates 16777597
17632570
17928336
18473783
18541215
18808677
19726522
19759518
20012528
20594957
24187576
24495362
25505066

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04640 Hematopoietic cell lineage - Homo sapiens (human)
hsa04658 Th1 and Th2 cell differentiation - Homo sapiens (human)
hsa04659 Th17 cell differentiation - Homo sapiens (human)
hsa04660 T cell receptor signaling pathway - Homo sapiens (human)
hsa05142 Chagas disease (American trypanosomiasis) - Homo sapiens (human)
hsa05162 Measles - Homo sapiens (human)
hsa05166 HTLV-I infection - Homo sapiens (human)
hsa05340 Primary immunodeficiency - Homo sapiens (human)
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