Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0000528
UniProt IDQ9UGN4
Primary gene name(s)CD300A
Synonym gene name(s)CMRF35H, IGSF12
Protein nameCMRF35-like molecule 8
Protein functionInhibitory receptor which may contribute to the down-regulation of cytolytic activity in natural killer, NK cells, and to the down-regulation of mast cell degranulation. {ECO:0000269|PubMed:10746781, ECO:0000269|PubMed:16339535, ECO:0000269|PubMed:9701027}.
Subcellular locationCell membrane {ECO:0000305};
Single-pass type I membrane protein {ECO:0000305}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q9UGN4
Gene Ontology
(Biological Process)
Complete annatation
cell adhesion [GO:0007155];
immune system process [GO:0002376];
negative regulation of activation of JAK2 kinase activity [GO:1902569];
negative regulation of B cell proliferation [GO:0030889];
negative regulation of B cell receptor signaling pathway [GO:0050859];
negative regulation of eosinophil activation [GO:1902567];
negative regulation of eosinophil migration [GO:2000417];
negative regulation of fibroblast proliferation [GO:0048147];
negative regulation of MAP kinase activity [GO:0043407];
negative regulation of mast cell activation involved in immune response [GO:0033007];
negative regulation of mast cell degranulation [GO:0043305];
negative regulation of neutrophil activation [GO:1902564];
negative regulation of NK T cell activation [GO:0051134];
negative regulation of phagocytosis, engulfment [GO:0060101];
positive regulation of phosphoprotein phosphatase activity [GO:0032516];
regulation of immune response [GO:0050776];
regulation of T cell receptor signaling pathway [GO:0050856]
Gene Ontology
(Molecular Function)
Complete annatation
phosphatidylethanolamine binding [GO:0008429];
phosphatidylserine binding [GO:0001786];
signaling receptor activity [GO:0038023]
Gene Ontology
(Cellular Component)
Complete annatation
extracellular exosome [GO:0070062];
integral component of membrane [GO:0016021];
membrane [GO:0016020];
plasma membrane [GO:0005886]
Protein-protein interaction116445
Phylogenetic treeQ9UGN4
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
      unknown
Brass et al., Science, 2008
      unknown
Smith et al., J. Immunol, 2010
      unknown
Interferon-stimulated
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model


DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3unknownunknownunknown
AZA vs. DISU-0.9331875575071450.003294970813903510.150510272875095
AZA vs. IL7-0.3437951621169940.3008633971588530.999311006273513
AZA vs. SAHAunknownunknownunknown
DISU vs. CD3unknownunknownunknown
DISU vs. IL7unknownunknownunknown
DISU vs. SAHAunknownunknownunknown
DMSO vs. AZA0.04872310815334280.8713683009370131
DMSO vs. CD3unknownunknownunknown
DMSO vs. DISU0.9782352112171410.001351699949914910.0751374351522434
DMSO vs. IL7-0.3796491361086380.2706769971912620.769026711771254
DMSO vs. SAHA-1.009032370560930.0008964395036344940.0166668712669096
HIV vs. Mock in Activationunknownunknownunknown
HIV vs. Mock in Latency-0.5428104461556760.003672131834415060.167179781442748
IL7 vs. CD3unknownunknownunknown
SAHA vs. CD3unknownunknownunknown
SAHA vs. IL7unknownunknownunknown
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.0763809 0.722222
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
2Q87 X-ray 1.7Å A/B/C=19-125.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Vif downregulates 23333304

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
not found
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