Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0000524
UniProt IDP20963
Primary gene name(s)CD247
Synonym gene name(s)CD3Z, T3Z, TCRZ
Protein nameT-cell surface glycoprotein CD3 zeta chain
Protein functionProbable role in assembly and expression of the TCR complex as well as signal transduction upon antigen triggering.
Subcellular locationMembrane;
Single-pass type I membrane protein.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P20963
Gene Ontology
(Biological Process)
Complete annatation
Fc-gamma receptor signaling pathway involved in phagocytosis [GO:0038096];
regulation of defense response to virus by virus [GO:0050690];
regulation of immune response [GO:0050776];
T cell costimulation [GO:0031295];
T cell receptor signaling pathway [GO:0050852];
viral process [GO:0016032]
Gene Ontology
(Molecular Function)
Complete annatation
identical protein binding [GO:0042802];
protein homodimerization activity [GO:0042803];
transmembrane signaling receptor activity [GO:0004888]
Gene Ontology
(Cellular Component)
Complete annatation
alpha-beta T cell receptor complex [GO:0042105];
cytoplasm [GO:0005737];
integral component of membrane [GO:0016021];
plasma membrane [GO:0005886];
T cell receptor complex [GO:0042101]
Protein-protein interaction107357
Phylogenetic treeP20963
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
      unknown
Brass et al., Science, 2008
      unknown
Smith et al., J. Immunol, 2010
      unknown
Interferon-stimulated
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model


DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.4608935595208190.1613357046794510.257726734597635
AZA vs. DISU-0.1045190014311460.6788155721719590.964884761087644
AZA vs. IL7-0.1194387671274750.5331430256958380.999311006273513
AZA vs. SAHA-0.3685137753578560.1324151188175080.463408864937525
DISU vs. CD30.3438799530064940.3436614512889080.476968300960224
DISU vs. IL7-0.02392314433391090.9241655655239730.985909727897307
DISU vs. SAHA-0.2628197116429840.3679242933160780.739899230381703
DMSO vs. AZA0.06855203043361630.6811249980629031
DMSO vs. CD30.5141090778723150.1094770902645820.183742464888139
DMSO vs. DISU0.1700823377509820.4848110105582820.902879625446528
DMSO vs. IL7-0.1802306739755440.3146361160574090.796193644348194
DMSO vs. SAHA-0.4429232659659550.06117718243001220.283954824885345
HIV vs. Mock in Activation-0.2093473353562710.7362665173581950.999983755607037
HIV vs. Mock in Latency-0.1413191181807250.3898868010690630.999834320637052
IL7 vs. CD30.3496584269295030.2776648364136890.413724997950133
SAHA vs. CD30.06749467177939530.8481974193809080.892496516552876
SAHA vs. IL7-0.2531360745652440.2995307861213650.548102704305883
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change -1.231877052
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.654139 0.000305467
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.991 0.974 1.027 1.02 1.147
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

Drugbank ID Drug Name Drug Status Pharmacological Action Drug Action
DB00075 Muromonab approved, investigational yes unknown

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
1TCE NMR - B=137-150.
1YGR X-ray 2.9Å C/D=80-85.
2HAC NMR - A/B=28-60.
2OQ1 X-ray 1.9Å B=69-87.
3IK5 X-ray 2.0Å B/D=63-80.
3IOZ X-ray 3.7Å B=51-93.
4XZ1 X-ray 2.8Å B=69-87.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Vpu interacts with 20179761
Nef interacts with 10224289
11463741
12734410
18438604
20179761
2284434511525746
12734410
16454711
18438604
capsid inhibits 7517794
Tat upregulates 18051367
Pr55(Gag) interacts with 25467409
8809126
9743208
Envelope surface glycoprotein gp120 induces phosphorylation of 19710135
capsid interacts with 1385321
2111780
7517794
8809126
9743208
107223708809126
9743208
Tat interacts with 9045614
9510190
17001685
Nef co-localizes with 22345473
matrix co-localizes with 25467409
Pr55(Gag) inhibits 1385321
2111780
7517794
8809126
9743208
107223707517794
Pr55(Gag) regulated by 22911005
Nef upregulates 15186530
16454711
18438604
22844345
23559827

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04650 Natural killer cell mediated cytotoxicity - Homo sapiens (human)
hsa04658 Th1 and Th2 cell differentiation - Homo sapiens (human)
hsa04659 Th17 cell differentiation - Homo sapiens (human)
hsa04660 T cell receptor signaling pathway - Homo sapiens (human)
hsa05142 Chagas disease (American trypanosomiasis) - Homo sapiens (human)
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